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Literature summary extracted from

  • Soror, S.H.; Rao, R.; Cullum, J.
    Mining the genome sequence for novel enzyme activity: characterisation of an unusual member of the hormone-sensitive lipase family of esterases from the genome of Streptomyces coelicolor A3 (2) (2009), Protein Eng. Des. Sel., 22, 333-339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.79 cloned and expressed as a His-tagged fusion protein in Escherichia coli Streptomyces coelicolor

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.79 V158A 4fold increase in activity with 4-nitrophenyl caproate, in contrast to wild-type mutant also shows activity against tributyrin. V158A mutant shows an improved thermostabilty compared with wild-type. V158A mutant shows a greater affinity to p-nitrophenyl acetate. Mutant is more sensitive to Cu2+, but less sensitive to Hg2+ compared to wild-type Streptomyces coelicolor
3.1.1.79 V158A/W87F significantly increased activity with 4-nitrophenyl butyrate in the double mutant, double mutant shows similar affinity to p-nitrophenyl acetate Streptomyces coelicolor
3.1.1.79 W87F mutation shows no effect on substrate specificity. Mutant is less thermostable compared to wild-type. V158A mutant shows an improved thermostabilty compared with wild-type. W87F mutant shows a lower affinity to p-nitrophenyl acetate compared to wild-type. Mutant is more sensitive to Cu2+ and Hg2+ compared to wild-type Streptomyces coelicolor

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.79 2-propanol 80-90% inhibition Streptomyces coelicolor
3.1.1.79 Cu2+ 10 mM, 50-60% inhibition Streptomyces coelicolor
3.1.1.79 ethanol 80-90% inhibition Streptomyces coelicolor
3.1.1.79 Hg2+ 10 mM, 50-60% inhibition Streptomyces coelicolor
3.1.1.79 methanol 80-90% inhibition Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.79 0.28
-
 4-nitrophenyl acetate mutant V158A, Vmax: 0.008 mM/min Streptomyces coelicolor
3.1.1.79 0.28
-
 p-nitrophenyl acetate mutant V158A, Vmax: 0.008 mM/min Streptomyces coelicolor
3.1.1.79 0.54
-
 1-naphthyl acetate Vmax: 0.019 mM/min Streptomyces coelicolor
3.1.1.79 0.54
-
 Alpha-naphtyl acetate Vmax: 0.019 mM/min Streptomyces coelicolor
3.1.1.79 1.4
-
 4-nitrophenyl acetate double mutant V158A/W87F, Vmax: 0.032 mM/min Streptomyces coelicolor
3.1.1.79 1.4
-
 p-nitrophenyl acetate double mutant V158A/W87F, Vmax: 0.032 mM/min Streptomyces coelicolor
3.1.1.79 1.71
-
 4-nitrophenyl acetate Vmax: 0.0094 mM/min Streptomyces coelicolor
3.1.1.79 1.71
-
 p-nitrophenyl acetate Vmax: 0.0094 mM/min Streptomyces coelicolor
3.1.1.79 5.43
-
 2-naphthyl acetate Vmax: 0.0168 mM/min Streptomyces coelicolor
3.1.1.79 7.42
-
 4-nitrophenyl acetate mutant W87F, Vmax: 0.0065 mM/min Streptomyces coelicolor
3.1.1.79 7.42
-
 p-nitrophenyl acetate mutant W87F, Vmax: 0.0065 mM/min Streptomyces coelicolor

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.79 K+ 10 M, 40% stimulation Streptomyces coelicolor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.79 38500
-
 calculated from cDNA Streptomyces coelicolor
3.1.1.79 38600
-
 SDS-PAGE Streptomyces coelicolor

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.79 Streptomyces coelicolor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.79 using Ni-NTA chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.79 1-naphthyl acetate + H2O
-
 Streptomyces coelicolor 1-naphthol + acetate
-
 ?
3.1.1.79 2-naphthyl acetate + H2O
-
 Streptomyces coelicolor 2-naphthol + acetate
-
 ?
3.1.1.79 4-nitrophenyl acetate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + acetate
-
 ?
3.1.1.79 4-nitrophenyl butyrate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + butyrate
-
 ?
3.1.1.79 4-nitrophenyl butyrate + H2O
-
 Streptomyces coelicolor ?
-
 ?
3.1.1.79 4-nitrophenyl caproate + H2O
-
 Streptomyces coelicolor 4-nitrophenol + caproate
-
 ?
3.1.1.79 4-nitrophenyl caproate + H2O
-
 Streptomyces coelicolor ?
-
 ?
3.1.1.79 alpha-naphtyl acetate + H2O
-
 Streptomyces coelicolor ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.79 HSL
-
 Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.79 37
-
 assay at Streptomyces coelicolor
3.1.1.79 55
-
 activity increases with temperature reaching a plateau at 55°C Streptomyces coelicolor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.79 55
-
 enzyme retains 70% of its activity after preincubation 1 h at 55°C Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.79 7
-
 assay at Streptomyces coelicolor
3.1.1.79 7.5
-
 optimal activity Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.79 6.5 8
-
 Streptomyces coelicolor

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.79 5.5 10 stable for 24 h at 20°C Streptomyces coelicolor