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Literature summary extracted from

  • Kiser, P.D.; Palczewski, K.
    Membrane-binding and enzymatic properties of RPE65 (2010), Prog. Retin. Eye Res., 29, 428-442.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.64 cytosol the ratio of cytosolic to microsomal RPE65 is approximately 1:2 Bos taurus 5829
-
3.1.1.64 membrane microsome Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.64 Fe2+ required Bos taurus
3.1.1.64 additional information Fe3+, Cu2+, Mg2+ and Zn2+ are unable to restore RPE65 isomerase activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.64 65000
-
x * 65000, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.64 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.64 purified from native bovine RPE microsomes in the presence of octyltetraoxyethylene. Triton X-100 and CHAPS are the most effective agents for extracting RPE65 from microsomal membranes Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.64 retinal pigment epithelial cell
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.64 all-trans-retinol
-
Bos taurus 11-cis-retinol
-
?
3.1.1.64 all-trans-retinyl ester + H2O
-
Bos taurus 11-cis-retinol + a fatty acid
-
?
3.1.1.64 additional information all-trans-9-des-methylretinol, retinyl acetate, retinyl heptanoate, all-trans-11-fluororetinol and all-trans-19,19,19-trifluororetinol are not isomerized Bos taurus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.64 ? x * 65000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.64 Rpe65
-
Bos taurus

General Information

EC Number General Information Comment Organism
3.1.1.64 physiological function RPE65 is involved not only for regeneration of rhodopsin but also plays an important role in regeneration of cone opsins and is critical for the health of cone photoreceptors. Interactions of RPE65 with an intact phospholipid bilayer are critical for its enzymatic activity Bos taurus