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Literature summary extracted from
- Racemization study on different N-acetylamino acids by a recombinant N-succinylamino acid racemase from Geobacillus kaustophilus CECT4264 (2009), Process Biochem., 44, 835-841.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.1.1.10 | over-expressed in Escherichia coli | Geobacillus kaustophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.10 | 8-hydroxy-5-quinolinesulfonic acid | - |
Geobacillus kaustophilus |  |
| 5.1.1.10 | beta-mercaptoethanol | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | dithiothreitol | - |
Geobacillus kaustophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.10 | 0.04 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.12 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.13 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.13 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | 3 | N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 5 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 7 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 8 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 17 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 18 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 27 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 41 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 43 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.1.1.10 | Co2+ | enhances activity, highest activity with Co2+ | Geobacillus kaustophilus | |
| 5.1.1.10 | Mn2+ | enhances activity | Geobacillus kaustophilus | |
| 5.1.1.10 | Ni2+ | enhances activity | Geobacillus kaustophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 43000 | - |
SDS-PAGE | Geobacillus kaustophilus |
| 5.1.1.10 | 150000 | - |
gel filtration, tetramer | Geobacillus kaustophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.1.10 | Geobacillus kaustophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.1.1.10 | purified in a one-step procedure by immobilized cobalt affinity chromatography | Geobacillus kaustophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.1.10 | N-acetyl-D-alanine | - |
Geobacillus kaustophilus | N-acetyl-L-alanine | - |
r | |
| 5.1.1.10 | N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | N-acetyl-L-asparagine | - |
r | |
| 5.1.1.10 | N-acetyl-D-methionine | - |
Geobacillus kaustophilus | N-acetyl-L-methionine | - |
r | |
| 5.1.1.10 | N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | N-acetyl-L-phenylalanine | - |
r | |
| 5.1.1.10 | N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | N-acetyl-L-tryptophan | - |
r | |
| 5.1.1.10 | N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | N-carbamoyl-D-methionine | - |
r | |
| 5.1.1.10 | N-succinyl-L-alanine | - |
Geobacillus kaustophilus | N-succinyl-D-alanine | - |
r | |
| 5.1.1.10 | N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | N-succinyl-D-phenylalanine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.1.1.10 | homotetramer | 4 * 43000, SDS-PAGE | Geobacillus kaustophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.1.1.10 | GkNSAAR | - |
Geobacillus kaustophilus |
| 5.1.1.10 | N-succinylamino acid racemase | - |
Geobacillus kaustophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 55 | - |
- |
Geobacillus kaustophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 60 | - |
GkNSAAR shows a gradual loss of activity at preincubation temperatures over 60°C | Geobacillus kaustophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.10 | 0.06 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.07 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.09 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.15 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.8 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 5 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 10 | - |
N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 15 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 16 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 20 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 22 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 43 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.1.1.10 | 8 | - |
- |
Geobacillus kaustophilus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.1.10 | 0.002 | - |
N-acetyl-L-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.004 | - |
N-acetyl-D-asparagine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.039 | - |
N-acetyl-L-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.047 | - |
N-acetyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.06 | - |
N-acetyl-L-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.061 | - |
N-acetyl-D-tryptophan | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.37 | - |
N-acetyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 0.467 | - |
N-acetyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 1.1 | - |
N-carbamoyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 1.2 | - |
N-carbamoyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 2.8 | - |
N-acetyl-L-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 3 | - |
N-acetyl-D-methionine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 35.1 | - |
N-succinyl-L-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 38.1 | - |
N-succinyl-D-phenylalanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 119 | - |
N-succinyl-D-alanine | - |
Geobacillus kaustophilus | |
| 5.1.1.10 | 352 | - |
N-succinyl-L-alanine | - |
Geobacillus kaustophilus | |
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