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Literature summary extracted from

  • Panagiotou, G.; Papadakis, M.; Topakas, E.; Olsson, L.; Christakopoulos, P.
    Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum (2008), Process Biochem., 43, 1114-1120.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.86 Ag2+ 1 mM Ag+ lowers the activity to 3% Fusarium oxysporum
2.7.1.86 beta-mercaptoethanol slightly inhibits NADH kinase Fusarium oxysporum
2.7.1.86 Ca2+ no activity in the presence of 10 mM Fusarium oxysporum
2.7.1.86 Cu2+ 1 mM Cu2+ lowers the activity to 33% Fusarium oxysporum
2.7.1.86 Fe2+ no activity in the presence of 10 mM Fusarium oxysporum
2.7.1.86 additional information NAD+ causes no inhibition of the enzyme Fusarium oxysporum
2.7.1.86 NADP+ slightly inhibits NADH kinase Fusarium oxysporum
2.7.1.86 Zn2+ 1 mM Zn2+ lowers the activity to 35% Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.86 0.13
-
 NADH
-
 Fusarium oxysporum
2.7.1.86 2.59
-
 ATP
-
 Fusarium oxysporum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.86 K+ K+ is the only monovalent metal ion resulting higher NADH kinase activity by almost 1.5fold Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.86 72000
-
 2 * 72000, gel filtration Fusarium oxysporum
2.7.1.86 156000
-
 gel filtration Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.86 Fusarium oxysporum
-
-
-
2.7.1.86 Fusarium oxysporum F3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.86 Q-Sepharose column chromatography, Sephacryl S300 gel filtration, SP-Sepharose column chromatography, and t-butyl hydrophobic interaction column chromatography Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.86 additional information
-
 the crude extract shows a specific activity of 0.017 units/mg, the 65.62fold purified enzyme shows a specific activity of 1.11 units/mg Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.86 ATP + NADH uses NADH as the preferred diphosphonicotinamide nucleotide donor Fusarium oxysporum ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH uses NADH as the preferred diphosphonicotinamide nucleotide donor Fusarium oxysporum F3 ADP + NADPH
-
 ?
2.7.1.86 additional information the purified enzyme presents almost no activity against NAD+ Fusarium oxysporum ?
-
 ?
2.7.1.86 additional information the purified enzyme presents almost no activity against NAD+ Fusarium oxysporum F3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.86 homodimer 2 * 72000, gel filtration Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.86 ATP-NADH kinase
-
 Fusarium oxysporum
2.7.1.86 ATP:NADH 2'-phosphotransferase
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.86 45
-
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.86 50
-
 the enzyme retains 8% of the activity after 1 h at 50°C Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.86 0.84
-
 ATP
-
 Fusarium oxysporum
2.7.1.86 0.95
-
 NADH
-
 Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.86 7.5
-
-
 Fusarium oxysporum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.86 6.5 8.5 less than 30% of the peak activity is displayed in pH 6.5 and 8.5 Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.86 5.5
-
 the enzyme is very unstable at pH values higher than 5.5 Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.86 ATP
-
 Fusarium oxysporum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.86 Fusarium oxysporum isoelectric focusing
-
 5.6