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Literature summary extracted from

  • Kourtoglou, E.; Mamma, D.; Topakas, E.; Christakopoulos, P.
    Purification, characterization and mass spectrometric sequencing of transaldolase from Fusarium oxysporum (2008), Process Biochem., 43, 1094-1101.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 0.49
-
 D-erythrose-4-phosphate
-
 Fusarium oxysporum
2.2.1.2 6.66
-
 D-fructose-6-phosphate
-
 Fusarium oxysporum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.2.1.2 36000
-
 SDS-PAGE, denatured Fusarium oxysporum
2.2.1.2 38000
-
 SDS-PAGE, purified Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Fusarium oxysporum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.2 anion exchange and gel filtration chromatographies, after last step 50fold purification Fusarium oxysporum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.2 0.3
-
 crude enzyme Fusarium oxysporum
2.2.1.2 15
-
 enzyme after purification with anion exchange and gel filtration chromatography Fusarium oxysporum

Storage Stability

EC Number Storage Stability Organism
2.2.1.2 30°C, 1 h, 20% activity lost Fusarium oxysporum
2.2.1.2 40°C, 1 h, 40% activity lost Fusarium oxysporum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-fructose 6-phosphate + D-erythrose 4-phosphate assay at 25°C, pH 7.5 Fusarium oxysporum glyceraldehyde 3-phosphate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.2.1.2 monomer
-
 Fusarium oxysporum

Synonyms

EC Number Synonyms Comment Organism
2.2.1.2 FoTal
-
 Fusarium oxysporum
2.2.1.2 transaldolase
-
 Fusarium oxysporum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.2.1.2 40 45
-
 Fusarium oxysporum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.2.1.2 30 50 at 30°C for 1 h only 80% residual activity, at 40°C for 1 h only 60% residual activity, at 50°C enzyme rapidly inactive, at 55°C about 30% activity lost Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.2 7.5
-
 imidazole buffer Fusarium oxysporum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.2.1.2 6 9 80% loss of activity with Tris/HCl buffer at pH 7.5, 95% loss of activity with Tris/HCl buffer at pH 9.0, 62% loss of activity with glycine/NaOH buffer at pH 9.0, about half of enzyme activity lost with phosphate buffer at pH 6.0 Fusarium oxysporum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.2.1.2 Fusarium oxysporum isoelectric focusing-PAGE
-
 4.5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.2 10.38
-
 D-fructose-6-phosphate
-
 Fusarium oxysporum
2.2.1.2 139.9
-
 D-erythrose-4-phosphate
-
 Fusarium oxysporum