BRENDA - Enzyme Database show

Identification and pharmacological characterization of cholesterol-5,6-epoxide hydrolase as a target for tamoxifen and AEBS ligands

de Medina, P.; Paillasse, M.R.; Segala, G.; Poirot, M.; Silvente-Poirot, S.; Proc. Natl. Acad. Sci. USA 107, 13520-13525 (2010)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.3.2.11
(11beta,17beta)-11-{4-[2-(Dimethylamino)ethoxy]phenyl}estra-1,3,5(10)-triene-3,17-diol
-
Rattus norvegicus
3.3.2.11
(4-(2-morpholinoethoxy)phenyl)(phenyl)methanone
-
Rattus norvegicus
3.3.2.11
(4-(6-(allyl (methyl)amino)hexyloxy)phenyl)(4-bromophenyl)methanone
-
Rattus norvegicus
3.3.2.11
(E)-N,3,6,6-tetra methyl-N-(naphthalene-1-ylmethyl)hept-2-en-4-yn-1-amine
-
Rattus norvegicus
3.3.2.11
(E)-N-(4-(3-chloro-4-cyclohexylphenyl)but-3-enyl)-N-ethylcyclohexanamine
-
Rattus norvegicus
3.3.2.11
1-(2-(4-(2-phenylpropan-2-yl) phenoxy)ethyl)pyrrolidine
-
Rattus norvegicus
3.3.2.11
1-(2-(4-benzylphenoxy)ethyl)-pyrrolidine
-
Rattus norvegicus
3.3.2.11
1-(4-(2-morpholino ethoxy)phenyl)-2-phenylethanone
-
Rattus norvegicus
3.3.2.11
1-[2-[4-[(Z)-1-(4-methoxyphenyl)-2-nitro-2-phenylethenyl]phenoxy]ethyl]pyrrolidine
-
Rattus norvegicus
3.3.2.11
2-(4-benzylphenoxy)-N,N-diethylethanamine
-
Rattus norvegicus
3.3.2.11
2-(4-chlorophenyl)-1-(4-(2-(diethylamino)ethoxy)phenyl)-1-p-tolyl ethanol
-
Rattus norvegicus
3.3.2.11
2-(diethylamino)ethyl 2,2-diphenylpentanoate
-
Rattus norvegicus
3.3.2.11
2-[4-[(Z)-2-chloro-1,2-di (phenyl)ethenyl]phenoxy]-N,N-diethylethanamine
-
Rattus norvegicus
3.3.2.11
3-beta-(2-(diethyl amino)ethoxy)androst-5-en-17-one
-
Rattus norvegicus
3.3.2.11
4-(2-(4-(2-phenylpropan-2-yl)phenoxy)ethyl)morpholine
-
Rattus norvegicus
3.3.2.11
4-(2-(4-benzylphenoxy)ethyl)morpholine
-
Rattus norvegicus
3.3.2.11
4-(4-(4-chloro phenyl)-4-hydroxypiperidin-1-yl)-1-(4-fluorophenyl)butan-1-one
-
Rattus norvegicus
3.3.2.11
4-[(Z)-1-[4-(2-dimethylaminoethyloxy)phenyl]-2-phenyl but-1-enyl]phenol
-
Rattus norvegicus
3.3.2.11
6-oxo-5-alpha-hydroxycholestanol
-
Rattus norvegicus
3.3.2.11
6-oxocholestanol
-
Rattus norvegicus
3.3.2.11
7-oxocholestanol
-
Rattus norvegicus
3.3.2.11
7-oxocholesterol
-
Rattus norvegicus
3.3.2.11
7alpha-hydroxycholesterol
-
Rattus norvegicus
3.3.2.11
7beta-hydroxycholesterol
-
Rattus norvegicus
3.3.2.11
AC-915
-
Rattus norvegicus
3.3.2.11
alpha-linolenic acid
-
Rattus norvegicus
3.3.2.11
amiodarone
-
Rattus norvegicus
3.3.2.11
arachidonic acid
-
Rattus norvegicus
3.3.2.11
cholestane-3beta,5alpha,6beta-triol
-
Rattus norvegicus
3.3.2.11
docosahexaenoic acid
-
Rattus norvegicus
3.3.2.11
ibogaine
-
Rattus norvegicus
3.3.2.11
N-(3,4-dichlorophenethyl)-N-methyl-2-(pyrrolidin-1-yl)ethanemine
-
Rattus norvegicus
3.3.2.11
oleic acid
-
Rattus norvegicus
3.3.2.11
phenyl{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}methanone
-
Rattus norvegicus
3.3.2.11
rimcazole
-
Rattus norvegicus
3.3.2.11
tamoxifen
0.01 mM, competitive
Rattus norvegicus
3.3.2.11
trans-1,4-bis(2-chlorobenzaminomethyl)cyclohexane
-
Rattus norvegicus
3.3.2.11
trifluoroperazine
-
Rattus norvegicus
3.3.2.11
[6-hydroxy-2-(4-hydroxyphenyl)-1-benzothiophen-3-yl]-[4-(2-piperidin-1-ylethoxy)phenyl]methanone
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.3.2.11
0.0074
-
5,6alpha-epoxy-5alpha-cholestan-3beta-ol
pH 7.4, 37C
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.3.2.11
microsome
-
Homo sapiens
-
-
3.3.2.11
microsome
-
Rattus norvegicus
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.3.2.11
Homo sapiens
-
-
-
3.3.2.11
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.3.2.11
liver
-
Rattus norvegicus
-
3.3.2.11
MCF-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.3.2.11
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
706552
Homo sapiens
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
706552
Rattus norvegicus
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
706552
Homo sapiens
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
706552
Rattus norvegicus
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.3.2.11
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.3.2.11
7.4
-
assay at
Rattus norvegicus
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.3.2.11
0.000006
-
(E)-N-(4-(3-chloro-4-cyclohexylphenyl)but-3-enyl)-N-ethylcyclohexanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000009
-
2-[4-[(Z)-2-chloro-1,2-di (phenyl)ethenyl]phenoxy]-N,N-diethylethanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000018
-
1-[2-[4-[(Z)-1-(4-methoxyphenyl)-2-nitro-2-phenylethenyl]phenoxy]ethyl]pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000027
-
1-(2-(4-benzylphenoxy)ethyl)-pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000027
-
4-(2-(4-benzylphenoxy)ethyl)morpholine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000034
-
tamoxifen
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000035
-
1-(2-(4-(2-phenylpropan-2-yl) phenoxy)ethyl)pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000036
-
[6-hydroxy-2-(4-hydroxyphenyl)-1-benzothiophen-3-yl]-[4-(2-piperidin-1-ylethoxy)phenyl]methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000039
-
2-(4-chlorophenyl)-1-(4-(2-(diethylamino)ethoxy)phenyl)-1-p-tolyl ethanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000057
-
4-(2-(4-(2-phenylpropan-2-yl)phenoxy)ethyl)morpholine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000062
-
2-(4-benzylphenoxy)-N,N-diethylethanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000089
-
(4-(6-(allyl (methyl)amino)hexyloxy)phenyl)(4-bromophenyl)methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.00009
-
3-beta-(2-(diethyl amino)ethoxy)androst-5-en-17-one
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000099
-
N-(3,4-dichlorophenethyl)-N-methyl-2-(pyrrolidin-1-yl)ethanemine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000135
-
trifluoroperazine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000145
-
4-[(Z)-1-[4-(2-dimethylaminoethyloxy)phenyl]-2-phenyl but-1-enyl]phenol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000155
-
(11beta,17beta)-11-{4-[2-(Dimethylamino)ethoxy]phenyl}estra-1,3,5(10)-triene-3,17-diol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000203
-
phenyl{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000241
-
(4-(2-morpholinoethoxy)phenyl)(phenyl)methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000649
-
trans-1,4-bis(2-chlorobenzaminomethyl)cyclohexane
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000733
-
amiodarone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000864
-
7-oxocholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000902
-
1-(4-(2-morpholino ethoxy)phenyl)-2-phenylethanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0019
-
2-(diethylamino)ethyl 2,2-diphenylpentanoate
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.002
-
ibogaine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0023
-
6-oxocholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0023
-
rimcazole
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0035
-
AC-915
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0042
-
7-oxocholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0061
-
7alpha-hydroxycholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0069
-
7beta-hydroxycholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0085
-
6-oxo-5-alpha-hydroxycholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0091
-
(E)-N,3,6,6-tetra methyl-N-(naphthalene-1-ylmethyl)hept-2-en-4-yn-1-amine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0097
-
cholestane-3beta,5alpha,6beta-triol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.012
-
docosahexaenoic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.018
-
4-(4-(4-chloro phenyl)-4-hydroxypiperidin-1-yl)-1-(4-fluorophenyl)butan-1-one
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.024
-
arachidonic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0363
-
alpha-linolenic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.054
-
oleic acid
pH 7.4, 37C
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.3.2.11
(11beta,17beta)-11-{4-[2-(Dimethylamino)ethoxy]phenyl}estra-1,3,5(10)-triene-3,17-diol
-
Rattus norvegicus
3.3.2.11
(4-(2-morpholinoethoxy)phenyl)(phenyl)methanone
-
Rattus norvegicus
3.3.2.11
(4-(6-(allyl (methyl)amino)hexyloxy)phenyl)(4-bromophenyl)methanone
-
Rattus norvegicus
3.3.2.11
(E)-N,3,6,6-tetra methyl-N-(naphthalene-1-ylmethyl)hept-2-en-4-yn-1-amine
-
Rattus norvegicus
3.3.2.11
(E)-N-(4-(3-chloro-4-cyclohexylphenyl)but-3-enyl)-N-ethylcyclohexanamine
-
Rattus norvegicus
3.3.2.11
1-(2-(4-(2-phenylpropan-2-yl) phenoxy)ethyl)pyrrolidine
-
Rattus norvegicus
3.3.2.11
1-(2-(4-benzylphenoxy)ethyl)-pyrrolidine
-
Rattus norvegicus
3.3.2.11
1-(4-(2-morpholino ethoxy)phenyl)-2-phenylethanone
-
Rattus norvegicus
3.3.2.11
1-[2-[4-[(Z)-1-(4-methoxyphenyl)-2-nitro-2-phenylethenyl]phenoxy]ethyl]pyrrolidine
-
Rattus norvegicus
3.3.2.11
2-(4-benzylphenoxy)-N,N-diethylethanamine
-
Rattus norvegicus
3.3.2.11
2-(4-chlorophenyl)-1-(4-(2-(diethylamino)ethoxy)phenyl)-1-p-tolyl ethanol
-
Rattus norvegicus
3.3.2.11
2-(diethylamino)ethyl 2,2-diphenylpentanoate
-
Rattus norvegicus
3.3.2.11
2-[4-[(Z)-2-chloro-1,2-di (phenyl)ethenyl]phenoxy]-N,N-diethylethanamine
-
Rattus norvegicus
3.3.2.11
3-beta-(2-(diethyl amino)ethoxy)androst-5-en-17-one
-
Rattus norvegicus
3.3.2.11
4-(2-(4-(2-phenylpropan-2-yl)phenoxy)ethyl)morpholine
-
Rattus norvegicus
3.3.2.11
4-(2-(4-benzylphenoxy)ethyl)morpholine
-
Rattus norvegicus
3.3.2.11
4-(4-(4-chloro phenyl)-4-hydroxypiperidin-1-yl)-1-(4-fluorophenyl)butan-1-one
-
Rattus norvegicus
3.3.2.11
4-[(Z)-1-[4-(2-dimethylaminoethyloxy)phenyl]-2-phenyl but-1-enyl]phenol
-
Rattus norvegicus
3.3.2.11
6-oxo-5-alpha-hydroxycholestanol
-
Rattus norvegicus
3.3.2.11
6-oxocholestanol
-
Rattus norvegicus
3.3.2.11
7-oxocholestanol
-
Rattus norvegicus
3.3.2.11
7-oxocholesterol
-
Rattus norvegicus
3.3.2.11
7alpha-hydroxycholesterol
-
Rattus norvegicus
3.3.2.11
7beta-hydroxycholesterol
-
Rattus norvegicus
3.3.2.11
AC-915
-
Rattus norvegicus
3.3.2.11
alpha-linolenic acid
-
Rattus norvegicus
3.3.2.11
amiodarone
-
Rattus norvegicus
3.3.2.11
arachidonic acid
-
Rattus norvegicus
3.3.2.11
cholestane-3beta,5alpha,6beta-triol
-
Rattus norvegicus
3.3.2.11
docosahexaenoic acid
-
Rattus norvegicus
3.3.2.11
ibogaine
-
Rattus norvegicus
3.3.2.11
N-(3,4-dichlorophenethyl)-N-methyl-2-(pyrrolidin-1-yl)ethanemine
-
Rattus norvegicus
3.3.2.11
oleic acid
-
Rattus norvegicus
3.3.2.11
phenyl{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}methanone
-
Rattus norvegicus
3.3.2.11
rimcazole
-
Rattus norvegicus
3.3.2.11
tamoxifen
0.01 mM, competitive
Rattus norvegicus
3.3.2.11
trans-1,4-bis(2-chlorobenzaminomethyl)cyclohexane
-
Rattus norvegicus
3.3.2.11
trifluoroperazine
-
Rattus norvegicus
3.3.2.11
[6-hydroxy-2-(4-hydroxyphenyl)-1-benzothiophen-3-yl]-[4-(2-piperidin-1-ylethoxy)phenyl]methanone
-
Rattus norvegicus
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.3.2.11
0.000006
-
(E)-N-(4-(3-chloro-4-cyclohexylphenyl)but-3-enyl)-N-ethylcyclohexanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000009
-
2-[4-[(Z)-2-chloro-1,2-di (phenyl)ethenyl]phenoxy]-N,N-diethylethanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000018
-
1-[2-[4-[(Z)-1-(4-methoxyphenyl)-2-nitro-2-phenylethenyl]phenoxy]ethyl]pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000027
-
1-(2-(4-benzylphenoxy)ethyl)-pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000027
-
4-(2-(4-benzylphenoxy)ethyl)morpholine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000034
-
tamoxifen
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000035
-
1-(2-(4-(2-phenylpropan-2-yl) phenoxy)ethyl)pyrrolidine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000036
-
[6-hydroxy-2-(4-hydroxyphenyl)-1-benzothiophen-3-yl]-[4-(2-piperidin-1-ylethoxy)phenyl]methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000039
-
2-(4-chlorophenyl)-1-(4-(2-(diethylamino)ethoxy)phenyl)-1-p-tolyl ethanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000057
-
4-(2-(4-(2-phenylpropan-2-yl)phenoxy)ethyl)morpholine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000062
-
2-(4-benzylphenoxy)-N,N-diethylethanamine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000089
-
(4-(6-(allyl (methyl)amino)hexyloxy)phenyl)(4-bromophenyl)methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.00009
-
3-beta-(2-(diethyl amino)ethoxy)androst-5-en-17-one
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000099
-
N-(3,4-dichlorophenethyl)-N-methyl-2-(pyrrolidin-1-yl)ethanemine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000135
-
trifluoroperazine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000145
-
4-[(Z)-1-[4-(2-dimethylaminoethyloxy)phenyl]-2-phenyl but-1-enyl]phenol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000155
-
(11beta,17beta)-11-{4-[2-(Dimethylamino)ethoxy]phenyl}estra-1,3,5(10)-triene-3,17-diol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000203
-
phenyl{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000241
-
(4-(2-morpholinoethoxy)phenyl)(phenyl)methanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000649
-
trans-1,4-bis(2-chlorobenzaminomethyl)cyclohexane
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000733
-
amiodarone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000864
-
7-oxocholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.000902
-
1-(4-(2-morpholino ethoxy)phenyl)-2-phenylethanone
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0019
-
2-(diethylamino)ethyl 2,2-diphenylpentanoate
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.002
-
ibogaine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0023
-
6-oxocholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0023
-
rimcazole
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0035
-
AC-915
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0042
-
7-oxocholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0061
-
7alpha-hydroxycholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0069
-
7beta-hydroxycholesterol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0085
-
6-oxo-5-alpha-hydroxycholestanol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0091
-
(E)-N,3,6,6-tetra methyl-N-(naphthalene-1-ylmethyl)hept-2-en-4-yn-1-amine
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0097
-
cholestane-3beta,5alpha,6beta-triol
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.012
-
docosahexaenoic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.018
-
4-(4-(4-chloro phenyl)-4-hydroxypiperidin-1-yl)-1-(4-fluorophenyl)butan-1-one
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.024
-
arachidonic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.0363
-
alpha-linolenic acid
pH 7.4, 37C
Rattus norvegicus
3.3.2.11
0.054
-
oleic acid
pH 7.4, 37C
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.3.2.11
0.0074
-
5,6alpha-epoxy-5alpha-cholestan-3beta-ol
pH 7.4, 37C
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.3.2.11
microsome
-
Homo sapiens
-
-
3.3.2.11
microsome
-
Rattus norvegicus
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.3.2.11
liver
-
Rattus norvegicus
-
3.3.2.11
MCF-7 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.3.2.11
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
706552
Homo sapiens
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
706552
Rattus norvegicus
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
706552
Homo sapiens
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
3.3.2.11
5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
706552
Rattus norvegicus
5alpha-cholestane-3beta,5alpha,6beta-triol
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.3.2.11
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.3.2.11
7.4
-
assay at
Rattus norvegicus
General Information
EC Number
General Information
Commentary
Organism
3.3.2.11
malfunction
knockdown of D8D7I (3beta-hydroxysterol-DELTA8-DELTA7-isomerase) and DHCR7 (3beta-hydroxysterol-DELTA7-reductase) abolishes ChEH activity. D8D7I and DHCR7 cooperate in the ChEH activity
Homo sapiens
3.3.2.11
physiological function
the existence of ChEH activity in MCF-7 cells, and thus ChEH inhibition and accumulation are likely to play a role in the mechanism of induction of breast cancer cell differentiation and apoptosis by antiestrogen binding site (AEBS) ligands that require sterol autoxidation products
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.3.2.11
malfunction
knockdown of D8D7I (3beta-hydroxysterol-DELTA8-DELTA7-isomerase) and DHCR7 (3beta-hydroxysterol-DELTA7-reductase) abolishes ChEH activity. D8D7I and DHCR7 cooperate in the ChEH activity
Homo sapiens
3.3.2.11
physiological function
the existence of ChEH activity in MCF-7 cells, and thus ChEH inhibition and accumulation are likely to play a role in the mechanism of induction of breast cancer cell differentiation and apoptosis by antiestrogen binding site (AEBS) ligands that require sterol autoxidation products
Homo sapiens