EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.1.25 | expression in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.1.1.9 | D-arginine | Pseudomonas aeruginosa | racemization of arginine is a two-step mechanism, intermediate is 2-keto-arginine, a first enzyme (DauA) catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and the second enzyme DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH, DauA and DauB are coupled catabolic and anabolic dehydrogenases to perform D-to-L racemization of arginine, which serves as prerequisite of D-arginine utilization through L-arginine catabolic pathways | L-arginine | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.1.25 | Pseudomonas aeruginosa | Q9HXE4 | - |
- |
1.4.1.25 | Pseudomonas aeruginosa DSM 22644 | Q9HXE4 | - |
- |
1.4.99.6 | Pseudomonas aeruginosa | Q9HXE3 | - |
- |
5.1.1.9 | Pseudomonas aeruginosa | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.1.25 | 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ | DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction | Pseudomonas aeruginosa | L-arginine + H2O + NAD(P)+ | - |
? | |
1.4.1.25 | 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ | DauB uses 2-ketoarginine and ammonia derived from D-arginine in the DauA-catalyzed reaction | Pseudomonas aeruginosa DSM 22644 | L-arginine + H2O + NAD(P)+ | - |
? | |
1.4.1.25 | L-arginine + H2O + NAD(P)+ | - |
Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ | - |
r | |
1.4.1.25 | L-arginine + H2O + NAD(P)+ | - |
Pseudomonas aeruginosa DSM 22644 | 5-guanidino-2-oxopentanoate + NH3 + NAD(P)H + H+ | - |
r | |
1.4.1.25 | additional information | no substantial reaction with D-arginine | Pseudomonas aeruginosa | ? | - |
? | |
1.4.1.25 | additional information | no substantial reaction with D-arginine | Pseudomonas aeruginosa DSM 22644 | ? | - |
? | |
5.1.1.9 | D-arginine | racemization of arginine is a two-step mechanism, intermediate is 2-keto-arginine, a first enzyme (DauA) catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and the second enzyme DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH, DauA and DauB are coupled catabolic and anabolic dehydrogenases to perform D-to-L racemization of arginine, which serves as prerequisite of D-arginine utilization through L-arginine catabolic pathways | Pseudomonas aeruginosa | L-arginine | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.1.25 | dauB | - |
Pseudomonas aeruginosa |
1.4.1.25 | NAD(P)H-dependent anabolic L-arginine dehydrogenase | - |
Pseudomonas aeruginosa |
1.4.99.6 | DauA | - |
Pseudomonas aeruginosa |
5.1.1.9 | arginine racemase | - |
Pseudomonas aeruginosa |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.4.1.25 | Pseudomonas aeruginosa | expression of the DauBAR operon is highly induced in presence of D-arginine | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.1.25 | physiological function | strains with a lesion at DauA or DauB genes fail to use D-arginine as sole carbon source. Growth complementation of an L-arginine auxotroph by D-arginine is abolished by a lesion at DauA or DauB. Presence of D-arginine induces L-arginine-specific genes in the parental strain PAO1 but not in its dauA or dauB mutants. DauA catalyzes oxidative deamination of D-arginine into 2-ketoarginine and ammonia, and DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH | Pseudomonas aeruginosa |
1.4.99.6 | physiological function | D-to-L inversion in D-arginine metabolism requires both D-arginine dehydrogenase DauA and NAD(P)H-dependent anabolic L-arginine dehydrogenase DauB. DauA catalyzes oxidative deamination of D-arginine into 2-oxoarginine and ammonia, and DauB is able to use 2-ketoarginine and ammonia as substrates and convert them into L-arginine in the presence of NADPH or NADH. DauA and DauB are coupled catabolic and anabolic dehydrogenases that enable D-arginine utilization through L-arginine catabolic pathways | Pseudomonas aeruginosa |