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Literature summary extracted from

  • Han, G.; Gupta, S.D.; Gable, K.; Niranjanakumari, S.; Moitra, P.; Eichler, F.; Brown, R.H.; Harmon, J.M.; Dunn, T.M.
    Identification of small subunits of mammalian serine palmitoyltransferase that confer distinct acyl-CoA substrate specificities (2009), Proc. Natl. Acad. Sci. USA, 106, 8186-8191.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.50 ssSPTa and ssSPTb, DNA and amino acid sequence determination and analysis. Transient functional overexpression of ssSPTb-containing isozymes in CHO LyB cells increasing C20-long-chain bases. Expression of the human enzyme subunits in the lcb1DELTAlcb2DELTA double mutant Saccharomyces cerevisiae cells in various combinations constituting enzyme activity, overview. Expression in CHO LyB cells lacking endogenous SPT activity Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.50 palmitoyl-CoA + L-serine Homo sapiens
-
 CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.50 Homo sapiens
-
 4 distinct SPT isozymes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.50 acyl-CoA + L-serine
-
 Homo sapiens CoA + ?
-
 ?
2.3.1.50 additional information ability of the ssSPT subunits to modulate the chain lengths of LCBs in mammalian cells Homo sapiens ?
-
 ?
2.3.1.50 palmitoyl-CoA + L-serine
-
 Homo sapiens CoA + 3-dehydro-D-sphinganine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.50 heterotrimer SPT is a heterodimer of 2 highly-related subunits, LCB1 and LCB2, existing in two isozyme forms LCB2a and LCB2b, associated with a third small activating subunit, either protein ssSPTa and protein ssSPTb, the proteins have a conserved hydrophobic central domain, reside in the membrane and interact with both hLCB1 and hLCB2 subunits, overview,protein ssSPTa and protein ssSPTb are required for maximal enzyme activity. SPT subunit interaction analysis, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.3.1.50 serine palmitoyltransferase
-
 Homo sapiens
2.3.1.50 SPT
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.50 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.50 8.1
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.50 pyridoxal 5'-phosphate
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.3.1.50 metabolism SPT catalyzes the first committed step in sphingolipid biosynthesis Homo sapiens