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Literature summary extracted from

  • Widhalm, J.R.; van Oostende, C.; Furt, F.; Basset, G.J.
    A dedicated thioesterase of the Hotdog-fold family is required for the biosynthesis of the naphthoquinone ring of vitamin K1 (2009), Proc. Natl. Acad. Sci. USA, 106, 5599-5603.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.28 expressed as a recombinant His-fusion protein Synechocystis sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.28 15478
-
 x * 15478, calculated from sequence Synechocystis sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.28 1,4-dihydroxy-2-naphthoyl-CoA + H2O Synechocystis sp. the reaction is involved in phylloquinone (vitamin K1) biosynthesis CoA + 1,4-dihydroxy-2-naphthoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.28 Synechocystis sp. Q55777 strain PCC 6803
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.2.28
-
 Synechocystis sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.28 1,4-dihydroxy-2-naphthoyl-CoA + H2O the reaction is involved in phylloquinone (vitamin K1) biosynthesis Synechocystis sp. CoA + 1,4-dihydroxy-2-naphthoate
-
 ?
3.1.2.28 1,4-dihydroxy-2-naphthoyl-CoA + H2O the enzyme is specific for 1,4-dihydroxy-2-naphthoyl-CoA and displays no activity against benzoyl-CoA, phenylacetyl-CoA, succinyl-CoA and palmitoyl-CoA Synechocystis sp. CoA + 1,4-dihydroxy-2-naphthoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.2.28 ? x * 15478, calculated from sequence Synechocystis sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.2.28 DHNA-CoA thioesterase
-
 Synechocystis sp.
3.1.2.28 slr0204
-
 Synechocystis sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.28 30
-
 assay at Synechocystis sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.28 7
-
 assay at Synechocystis sp.

General Information

EC Number General Information Comment Organism
3.1.2.28 malfunction knockout mutant accumulates 1,4-dihydroxy-2-naphthoyl-CoA, contains 13fold less phylloquinone than the wild-type and displays typical photosensitivity to high light intensities associated with phylloquinone deficiency in cyanobacteria Synechocystis sp.
3.1.2.28 physiological function the reaction is involved in phylloquinone (vitamin K1) biosynthesis Synechocystis sp.