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Literature summary extracted from

  • Raunser, S.; Magnani, R.; Huang, Z.; Houtz, R.L.; Trievel, R.C.; Penczek, P.A.; Walz, T.
    Rubisco in complex with Rubisco large subunit methyltransferase (2009), Proc. Natl. Acad. Sci. USA, 106, 3160-3165.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.127 RLSMT, expression in Escherichia coli Pisum sativum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.39 cryoelectron microscopy is used to produce an 11-A density map of the Rubisco-Rubisco large subunit methyltransferase (RLSMT) complex. The atomic model of the complex, obtained by fitting crystal structures of Rubisco and Rubisco large subunit methyltransferase into the density map, shows that the extensive contact regions between the 2 proteins are mainly mediated by hydrophobic residues and leucine-rich repeats Spinacia oleracea
4.1.1.39 cryoelectron microscopy is used to produce an 11-A density map of the Rubisco-Rubisco large subunit methyltransferase (RLSMT) complex. The atomic model of the complex, obtained by fitting crystal structures of Rubisco and Rubisco large subunit methyltransferase into the density map, shows that the extensive contact regions between the 2 proteins are mainly mediated by hydrophobic residues and leucine-rich repeats Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.127 additional information
-
 additional information substrate binding kinetics Pisum sativum
2.1.1.127 0.0014
-
 [ribulose-1,5-bisphosphate carboxylase]-lysine
-
 Pisum sativum
2.1.1.127 0.006
-
 S-adenosyl-L-methionine
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.127 S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate carboxylase]-lysine Pisum sativum LSMT transfers one or two methyl groups to a single lysine S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate carboxylase]-N6-methyl-L-lysine
-
 ?
4.1.1.39 additional information Spinacia oleracea Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco) ?
-
 ?
4.1.1.39 additional information Nicotiana tabacum Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco) ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.127 Pisum sativum
-
-
-
4.1.1.39 Nicotiana tabacum
-
-
-
4.1.1.39 Spinacia oleracea
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.127 3 S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate carboxylase]-L-lysine = 3 S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate carboxylase]-N6,N6,N6-trimethyl-L-lysine substrate binding, leucine-rich motifscontribute to the contact surface with Rubisco, overview Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.39 leaf
-
 Spinacia oleracea
-
4.1.1.39 leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.127 S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate carboxylase]-lysine LSMT transfers one or two methyl groups to a single lysine Pisum sativum S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate carboxylase]-N6-methyl-L-lysine
-
 ?
2.1.1.127 S-adenosyl-L-methionine + [ribulose-1,5-bisphosphate carboxylase]-lysine Rubisco from spinach or tobacco leaves. LSMT transfers one or two methyl groups to a single lysine Pisum sativum S-adenosyl-L-homocysteine + [ribulose-1,5-bisphosphate carboxylase]-N6-methyl-L-lysine
-
 ?
4.1.1.39 additional information Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco) Spinacia oleracea ?
-
 ?
4.1.1.39 additional information Rubisco is in complex with Pisum sativum Rubisco large subunit methyltransferase (responsible for the formation of trimethyl-lysine-14 in the large subunit of Rubisco) Nicotiana tabacum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.127 RLSMT
-
 Pisum sativum
2.1.1.127 Rubisco large subunit methyltransferase
-
 Pisum sativum
4.1.1.39 Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Spinacia oleracea
4.1.1.39 Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Nicotiana tabacum
4.1.1.39 Rubisco
-
 Spinacia oleracea
4.1.1.39 Rubisco
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.127 S-adenosyl-L-methionine
-
 Pisum sativum