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Literature summary extracted from

  • Zaunbrecher, M.A.; Sikes, R.D.; Metchock, B.; Shinnick, T.M.; Posey, J.E.
    Overexpression of the chromosomally encoded aminoglycoside acetyltransferase eis confers kanamycin resistance in Mycobacterium tuberculosis (2009), Proc. Natl. Acad. Sci. USA, 106, 20004-20009.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.82 medicine a C-14T mutation in eis confers kanamycin resistance in Mycobacterium tuberculosis Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.82 into the vector pET19b Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.82 0.112
-
 amikacin
-
 Mycobacterium tuberculosis
2.3.1.82 0.154
-
 kanamycin
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.82 acetyl-CoA + aminoglycoside Mycobacterium tuberculosis
-
 CoA + 6'-N-acetylaminoglycoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.82 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.82 on a His-Bind NiNTA column Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.82 acetyl-CoA + amikacin aminoglycoside acetyltransferase eis acetylates and inactivates amikacin Mycobacterium tuberculosis CoA + 6'-N-acetylamikacin
-
 ?
2.3.1.82 acetyl-CoA + aminoglycoside
-
 Mycobacterium tuberculosis CoA + 6'-N-acetylaminoglycoside
-
 ?
2.3.1.82 acetyl-CoA + kanamycin aminoglycoside acetyltransferase eis acetylates and inactivates kanamycin Mycobacterium tuberculosis CoA + 6'-N-acetylkanamycin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.82 aminoglycoside acetyltransferase eis
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.82 22
-
 activity assay at room temperature Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.82 8
-
 activity assay Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.82 acetyl-CoA
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
2.3.1.82 Mycobacterium tuberculosis C-14T mutation in eis increases expression of aminoglycoside acetyltransferase eis up