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Literature summary extracted from

  • Nagao, A.; Suzuki, T.; Katoh, T.; Sakaguchi, Y.; Suzuki, T.
    Biogenesis of glutaminyl-mt tRNAGln in human mitochondria (2009), Proc. Natl. Acad. Sci. USA, 106, 16209-16214.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 0.00027
-
 tRNAGlu
-
 Homo sapiens
6.1.1.17 0.0017
-
 tRNAGln
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.17 mitochondrion
-
 Homo sapiens 5739
-
6.3.5.7 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.7 132000
-
 gel filtration, GatABC complex Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Homo sapiens
-
-
-
6.3.5.7 Homo sapiens O43716 subunit C
-
6.3.5.7 Homo sapiens O75879 subunit B
-
6.3.5.7 Homo sapiens Q9H0R6 subunit A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGln mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu Homo sapiens AMP + diphosphate + L-glutamyl-tRNAGln
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu Homo sapiens AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.3.5.7 ATP + Glu-tRNAGln + L-glutamine
-
 Homo sapiens ADP + phosphate + Gln-tRNAGln + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.7 trimer 1 * subunit A plus 1 * subunit B plus 1 * subunit C Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.17 0.31
-
 tRNAGlu
-
 Homo sapiens
6.1.1.17 0.95
-
 tRNAGln
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
6.3.5.7 physiological function inactivation of any of the hGatCAB subunits by siRNA-mediated knock down in HeLa cells leads to accumulation of the Glu-charged form of tRNAGln and defects in respiration can be observed Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.17 560
-
 tRNAGln
-
 Homo sapiens
6.1.1.17 1148
-
 tRNAGlu
-
 Homo sapiens