BRENDA - Enzyme Database

Biogenesis of glutaminyl-mt tRNAGln in human mitochondria

Nagao, A.; Suzuki, T.; Katoh, T.; Sakaguchi, Y.; Suzuki, T.; Proc. Natl. Acad. Sci. USA 106, 16209-16214 (2009)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00027
-
tRNAGlu
-
Homo sapiens
6.1.1.17
0.0017
-
tRNAGln
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
mitochondrion
-
Homo sapiens
5739
-
6.3.5.7
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
132000
-
gel filtration, GatABC complex; gel filtration, GatABC complex; gel filtration, GatABC complex
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Homo sapiens
-
-
-
6.3.5.7
Homo sapiens
O43716
subunit C
-
6.3.5.7
Homo sapiens
O75879
subunit B
-
6.3.5.7
Homo sapiens
Q9H0R6
subunit A
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGln
mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu
706514
Homo sapiens
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu
706514
Homo sapiens
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
706514
Homo sapiens
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.7
trimer
1 * subunit A plus 1 * subunit B plus 1 * subunit C; 1 * subunit A plus 1 * subunit B plus 1 * subunit C; 1 * subunit A plus 1 * subunit B plus 1 * subunit C
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.31
-
tRNAGlu
-
Homo sapiens
6.1.1.17
0.95
-
tRNAGln
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.00027
-
tRNAGlu
-
Homo sapiens
6.1.1.17
0.0017
-
tRNAGln
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.1.1.17
mitochondrion
-
Homo sapiens
5739
-
6.3.5.7
mitochondrion
-
Homo sapiens
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.3.5.7
132000
-
gel filtration, GatABC complex
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGln
mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu
706514
Homo sapiens
AMP + diphosphate + L-glutamyl-tRNAGln
-
-
-
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
mitochondrial glutamyl-tRNA synthetase efficiently aminoacylates both tRNAGln to form Glu-tRNAGln and tRNAGlu to form Glu-tRNAGlu
706514
Homo sapiens
AMP + diphosphate + L-glutamyl-tRNAGlu
-
-
-
?
6.3.5.7
ATP + Glu-tRNAGln + L-glutamine
-
706514
Homo sapiens
ADP + phosphate + Gln-tRNAGln + L-glutamate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.7
trimer
1 * subunit A plus 1 * subunit B plus 1 * subunit C
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
0.31
-
tRNAGlu
-
Homo sapiens
6.1.1.17
0.95
-
tRNAGln
-
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
6.3.5.7
physiological function
inactivation of any of the hGatCAB subunits by siRNA-mediated knock down in HeLa cells leads to accumulation of the Glu-charged form of tRNAGln and defects in respiration can be observed; inactivation of any of the hGatCAB subunits by siRNA-mediated knock down in HeLa cells leads to accumulation of the Glu-charged form of tRNAGln and defects in respiration can be observed; inactivation of any of the hGatCAB subunits by siRNA-mediated knock down in HeLa cells leads to accumulation of the Glu-charged form of tRNAGln and defects in respiration can be observed
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
6.3.5.7
physiological function
inactivation of any of the hGatCAB subunits by siRNA-mediated knock down in HeLa cells leads to accumulation of the Glu-charged form of tRNAGln and defects in respiration can be observed
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.1.1.17
560
-
tRNAGln
-
Homo sapiens
6.1.1.17
1148
-
tRNAGlu
-
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.1.1.17
560
-
tRNAGln
-
Homo sapiens
6.1.1.17
1148
-
tRNAGlu
-
Homo sapiens