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Literature summary extracted from

  • Wang, W.; Hu, T.; Frantom, P.A.; Zheng, T.; Gerwe, B.; Del Amo, D.S.; Garret, S.; Seidel, R.D.; Wu, P.
    Chemoenzymatic synthesis of GDP-L-fucose and the Lewis X glycan derivatives (2009), Proc. Natl. Acad. Sci. USA, 106, 16096-16101.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.152 synthesis a chemoenzymatic model for the preparative-scale synthesis of a diverse array of GDP-fucose derivatives is reported Helicobacter pylori
2.7.7.30 synthesis a chemoenzymatic model for the preparative-scale synthesis of a diverse array of GDP-fucose derivatives is reported Bacteroides fragilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.152 into the vector pET-24b+ for expression in Escherichia coli cells Helicobacter pylori
2.7.7.30 into the vector pET-16b for expression in Escherichia coli cells Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.30 0.012
-
 GTP
-
 Bacteroides fragilis
2.7.7.30 0.03
-
 beta-L-fucose 1-phosphate
-
 Bacteroides fragilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.30 additional information requires the presence of divalent metal cations at the active site Bacteroides fragilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.30 GTP + beta-L-fucose 1-phosphate Bacteroides fragilis
-
 diphosphate + GDP-L-fucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.152 Helicobacter pylori O25366
-
-
2.7.7.30 Bacteroides fragilis
-
 Bacteroides fragilis 9343
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.152 GDP-6,7-dideoxy-beta-L-galacto-hept-6-enopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl 6,7-dideoxy-beta-L-galacto-hept-6-enopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-6-azido-6-deoxy-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl -6-azido-6-deoxy-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-6-deoxy-6-fluoro-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl -6-deoxy-6-fluoro-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-6-O-methyl-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl 6-O-methyl-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-alpha-D-(5-cyano)arabinopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl alpha-D-(5-cyano)arabinopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-alpha-D-arabinopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl alpha-D-arabinopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-beta-L-galactopyranose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl beta-L-galactopyranosyl-(1->3)-[beta-D-galactopyranosyl-(1->4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.4.1.152 GDP-L-fucose + 2-azidoethyl 2-(acetylamino)-2-deoxy-4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
 Helicobacter pylori GDP + 2-azidoethyl 6-deoxy-beta-L-galactopyranosyl-(1-3)-[beta-D-galactopyranosyl-(1-4)]-2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
-
 ?
2.7.7.30 GTP + 6,7-dideoxy-beta-L-galacto-hept-6-enopyranose 1-phosphate
-
 Bacteroides fragilis GDP-6,7-dideoxy-beta-L-galacto-hept-6-enopyranose + diphosphate
-
 ?
2.7.7.30 GTP + 6-azido-6-deoxy-beta-L-galactopyranose 1-phosphate
-
 Bacteroides fragilis GDP-6-azido-6-deoxy-beta-L-galactopyranose + diphosphate
-
 ?
2.7.7.30 GTP + 6-deoxy-6-fluoro-beta-L-galactopyranose 1-phosphate
-
 Bacteroides fragilis GDP-6-deoxy-6-fluoro-beta-L-galactopyranose + diphosphate
-
 ?
2.7.7.30 GTP + 6-O-methyl-beta-L-galactopyranose 1-phosphate
-
 Bacteroides fragilis GDP-6-O-methyl-beta-L-galactopyranose + diphosphate
-
 ?
2.7.7.30 GTP + alpha-D-(5-cyano)arabinopyranose 1-phosphate
-
 Bacteroides fragilis GDP-alpha-D-(5-cyano)arabinopyranose + diphosphate
-
 ?
2.7.7.30 GTP + alpha-D-arabinopyranose 1-phosphate
-
 Bacteroides fragilis GDP-alpha-D-arabinopyranose + diphosphate
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis diphosphate + GDP-L-fucose
-
 ?
2.7.7.30 GTP + beta-L-fucose 1-phosphate
-
 Bacteroides fragilis GDP-L-fucose + diphosphate
-
 ?
2.7.7.30 GTP + beta-L-galactopyranose 1-phosphate
-
 Bacteroides fragilis GDP-beta-L-galactopyranose + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.152 alpha1,3 fucosyltransferase
-
 Helicobacter pylori
2.7.7.30 FKP
-
 Bacteroides fragilis
2.7.7.30 GDP-fucose pyrophosphorylase
-
 Bacteroides fragilis
2.7.7.30 L-fucokinase/GDP-fucose pyrophosphorylase bifunctional enzyme Bacteroides fragilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.30 37
-
 activity assay Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.30 7.5
-
 activity assay Bacteroides fragilis