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Literature summary extracted from

  • Schilmiller, A.L.; Schauvinhold, I.; Larson, M.; Xu, R.; Charbonneau, A.L.; Schmidt, A.; Wilkerson, C.; Last, R.L.; Pichersky, E.
    Monoterpenes in the glandular trichomes of tomato are synthesized from a neryl diphosphate precursor rather than geranyl diphosphate (2009), Proc. Natl. Acad. Sci. USA, 106, 10865-10870.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.51 the complete ORF of PHS1 encodes a plastid transit peptide, construction of an ORF of PHS1 that begins at Met45 and includes a His-tag extension at the N-terminus, expression in Eschreichia coli Solanum lycopersicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.28 0.152
-
isopentenyl diphosphate pH 8.0, 30°C Solanum lycopersicum
2.5.1.28 0.177
-
dimethylallyl diphosphate pH 8.0, 30°C Solanum lycopersicum
4.2.3.51 0.0091
-
neryl diphosphate pH 8.0, 30°C Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.51 5 neryl diphosphate Solanum lycopersicum
-
5 diphosphate + alpha-phellandrene + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.28 Solanum lycopersicum C1K5M2
-
-
4.2.3.51 Solanum lycopersicum C1K5M3 PHS1 maps to chromosome 8; cultivar M82
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.28 trichome glandular trichome Solanum lycopersicum
-
4.2.3.51 secretory trichome type VI glandular trichome, highly expressed Solanum lycopersicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.28 dimethylallyl diphosphate + isopentenyl diphosphate
-
Solanum lycopersicum diphosphate + neryl diphosphate
-
?
4.2.3.51 5 neryl diphosphate
-
Solanum lycopersicum 5 diphosphate + alpha-phellandrene + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes ?
4.2.3.51 additional information the enzyme has very poor affinity for geranyl diphosphate Solanum lycopersicum ?
-
?
4.2.3.51 neryl diphosphate neryl diphosphate most likely ionizes to a neryl cation, which can further isomerize to an alpha-terpinyl cation. Further transformations of this cyclic intermediate can eventually yield all observed products of PHS1 Solanum lycopersicum diphosphate + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene + alpha-phellandrene the main product is beta-phellandrene ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.28 neryl diphosphate synthase
-
Solanum lycopersicum
4.2.3.51 monoterpene synthase PHS1
-
Solanum lycopersicum
4.2.3.51 phellandrene synthase 1
-
Solanum lycopersicum
4.2.3.51 PHS1
-
Solanum lycopersicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.28 0.2
-
dimethylallyl diphosphate pH 8.0, 30°C Solanum lycopersicum
4.2.3.51 4.1
-
neryl diphosphate pH 8.0, 30°C Solanum lycopersicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.28 1.04
-
dimethylallyl diphosphate pH 8.0, 30°C Solanum lycopersicum
2.5.1.28 1.39
-
isopentenyl diphosphate pH 8.0, 30°C Solanum lycopersicum
4.2.3.51 451.5
-
neryl diphosphate pH 8.0, 30°C Solanum lycopersicum