EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.51 | the complete ORF of PHS1 encodes a plastid transit peptide, construction of an ORF of PHS1 that begins at Met45 and includes a His-tag extension at the N-terminus, expression in Eschreichia coli | Solanum lycopersicum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.28 | 0.152 | - |
isopentenyl diphosphate | pH 8.0, 30°C | Solanum lycopersicum | |
2.5.1.28 | 0.177 | - |
dimethylallyl diphosphate | pH 8.0, 30°C | Solanum lycopersicum | |
4.2.3.51 | 0.0091 | - |
neryl diphosphate | pH 8.0, 30°C | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.51 | 5 neryl diphosphate | Solanum lycopersicum | - |
5 diphosphate + alpha-phellandrene + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene | main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.28 | Solanum lycopersicum | C1K5M2 | - |
- |
4.2.3.51 | Solanum lycopersicum | C1K5M3 | PHS1 maps to chromosome 8; cultivar M82 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.28 | trichome | glandular trichome | Solanum lycopersicum | - |
4.2.3.51 | secretory trichome | type VI glandular trichome, highly expressed | Solanum lycopersicum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.28 | dimethylallyl diphosphate + isopentenyl diphosphate | - |
Solanum lycopersicum | diphosphate + neryl diphosphate | - |
? | |
4.2.3.51 | 5 neryl diphosphate | - |
Solanum lycopersicum | 5 diphosphate + alpha-phellandrene + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene | main product is beta-phellandrene. The profile of monoterpenes produced by PHS1 is identical with the monoterpenes found in type VI glandular trichome. In tomato glands, neryl diphosphate serves as a precursor for the synthesis of monoterpenes | ? | |
4.2.3.51 | additional information | the enzyme has very poor affinity for geranyl diphosphate | Solanum lycopersicum | ? | - |
? | |
4.2.3.51 | neryl diphosphate | neryl diphosphate most likely ionizes to a neryl cation, which can further isomerize to an alpha-terpinyl cation. Further transformations of this cyclic intermediate can eventually yield all observed products of PHS1 | Solanum lycopersicum | diphosphate + beta-phellandrene + alpha-terpinene + beta-terpinene + limonene + alpha-phellandrene | the main product is beta-phellandrene | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.28 | neryl diphosphate synthase | - |
Solanum lycopersicum |
4.2.3.51 | monoterpene synthase PHS1 | - |
Solanum lycopersicum |
4.2.3.51 | phellandrene synthase 1 | - |
Solanum lycopersicum |
4.2.3.51 | PHS1 | - |
Solanum lycopersicum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.28 | 0.2 | - |
dimethylallyl diphosphate | pH 8.0, 30°C | Solanum lycopersicum | |
4.2.3.51 | 4.1 | - |
neryl diphosphate | pH 8.0, 30°C | Solanum lycopersicum |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.28 | 1.04 | - |
dimethylallyl diphosphate | pH 8.0, 30°C | Solanum lycopersicum | |
2.5.1.28 | 1.39 | - |
isopentenyl diphosphate | pH 8.0, 30°C | Solanum lycopersicum | |
4.2.3.51 | 451.5 | - |
neryl diphosphate | pH 8.0, 30°C | Solanum lycopersicum |