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Literature summary extracted from

  • Nocek, B.; Kochinyan, S.; Proudfoot, M.; Brown, G.; Evdokimova, E.; Osipiuk, J.; Edwards, A.M.; Savchenko, A.; Joachimiak, A.; Yakunin, A.F.
    Polyphosphate-dependent synthesis of ATP and ADP by the family-2 polyphosphate kinases in bacteria (2008), Proc. Natl. Acad. Sci. USA, 105, 17730-17735.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) Pseudomonas aeruginosa
2.7.4.1 expressed in Escherichia coli strains BL21 (DE3) and B834 (DE3) Sinorhizobium meliloti

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.1 hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 7.5), 0.1 M Na-formate, 0.1 M Li-sulfate, and 0.3 M NDSB 211 Sinorhizobium meliloti
2.7.4.1 hanging drop vapor diffusion method, using 0.1 M HEPES-K (pH 7.0), 5% tacsimate, and 10% PEG MME 5K Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 D148A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 D223A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 D307A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 D320A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 D323A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 D362A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 D437A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 D93A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 E304A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 E90A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 K311A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 K473A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 K97A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 Q202A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 Q416A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R149A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 R205A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 R209A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 R263A the mutant shows almost wild type activity Sinorhizobium meliloti
2.7.4.1 R316A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R325A the mutant shows reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R363A the mutant shows almost no activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R419A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R423A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 R477A the mutant shows increased activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 W194A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 W408A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa
2.7.4.1 Y233A the mutant shows strongly reduced activity compared to the wild type enzyme Sinorhizobium meliloti
2.7.4.1 Y447A the mutant shows strongly reduced activity compared to the wild type enzyme Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ Pseudomonas aeruginosa
2.7.4.1 Mg2+ no activity in the absence of a divalent metal cation, Mg2+ is the most effective metal, whereas low activity is observed with Co2+ or Ni2+ and no activity is observed in the presence of Mn2+ or Ca2+ Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.1 97000
-
 gel filtration Pseudomonas aeruginosa
2.7.4.1 124500
-
 gel filtration Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Pseudomonas aeruginosa
-
-
-
2.7.4.1 Sinorhizobium meliloti Q92SA6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1
-
 Pseudomonas aeruginosa
2.7.4.1
-
 Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
 Pseudomonas aeruginosa ADP + (phosphate)n+1
-
 ?
2.7.4.1 ATP + (phosphate)n
-
 Sinorhizobium meliloti ADP + (phosphate)n+1
-
 ?
2.7.4.1 additional information PA3455 protein is also active with (phosphate)3 as a phosphodonor, but its activity and substrate affinity are lower than those with (phosphate)12-13 Pseudomonas aeruginosa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.1 homodimer x-ray crystallography Pseudomonas aeruginosa
2.7.4.1 homotetramer x-ray crystallography Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 family-2 polyphosphate kinase
-
 Pseudomonas aeruginosa
2.7.4.1 family-2 polyphosphate kinase
-
 Sinorhizobium meliloti
2.7.4.1 PA3455 protein
-
 Pseudomonas aeruginosa
2.7.4.1 PPK2
-
 Pseudomonas aeruginosa
2.7.4.1 PPK2
-
 Sinorhizobium meliloti
2.7.4.1 SMc02148 protein
-
 Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.1 8 9.5
-
 Pseudomonas aeruginosa
2.7.4.1 8 9.5
-
 Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 ATP
-
 Pseudomonas aeruginosa
2.7.4.1 ATP
-
 Sinorhizobium meliloti