BRENDA - Enzyme Database

Dissociation of the octameric enolase from S. pyogenes--one interface stabilizes another

Karbassi, F.; Quiros, V.; Pancholi, V.; Kornblatt, M.J.; PLoS ONE 5, e8810 (2010)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Streptococcus pyogenes
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
F137L/E363G
the dimer-dimer interface mutant destabilizes the octameric structure, the double mutant is more easily dissociated in the presence of NaClO4 than is the wild type
Streptococcus pyogenes
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
NaClO4
enolase at 19.4 mM after incubation in 0.2 M NaClO4, has 32% of its original activity and is 21% octameric. Following a 24 h dialysis against buffer, the protein is 77% octameric and has 74% of its original activity
Streptococcus pyogenes
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
452000
-
calculated molecular mass
Streptococcus pyogenes
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Streptococcus pyogenes
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography
Streptococcus pyogenes
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
706454
Streptococcus pyogenes
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
sedimentation velocity analysis
Streptococcus pyogenes
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.11
60
-
mutant enzyme F137L/E363G, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2
Streptococcus pyogenes
NaClO4
4.2.1.11
200
-
wild type enzyme, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2
Streptococcus pyogenes
NaClO4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Streptococcus pyogenes
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
F137L/E363G
the dimer-dimer interface mutant destabilizes the octameric structure, the double mutant is more easily dissociated in the presence of NaClO4 than is the wild type
Streptococcus pyogenes
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.2.1.11
60
-
mutant enzyme F137L/E363G, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2
Streptococcus pyogenes
NaClO4
4.2.1.11
200
-
wild type enzyme, in 50 mM HEPES, pH 7.5, 1 mM Mg(OAc)2
Streptococcus pyogenes
NaClO4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
NaClO4
enolase at 19.4 mM after incubation in 0.2 M NaClO4, has 32% of its original activity and is 21% octameric. Following a 24 h dialysis against buffer, the protein is 77% octameric and has 74% of its original activity
Streptococcus pyogenes
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
452000
-
calculated molecular mass
Streptococcus pyogenes
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA column chromatography
Streptococcus pyogenes
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
706454
Streptococcus pyogenes
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
octamer
sedimentation velocity analysis
Streptococcus pyogenes