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Literature summary extracted from

  • Chen, H.; Contreras, X.; Yamaguchi, Y.; Handa, H.; Peterlin, B.M.; Guo, S.
    Repression of RNA polymerase II elongation in vivo is critically dependent on the C-terminus of Spt5 (2009), PLoS ONE, 4, e6918.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.6 Spt5 the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.6 Spt5 the large subunit of the DRB sensitivity-inducing factor, DSIF, represses or activates RNAPII elongation in vitro. CTR1 and CTR2CT, the two repeat-containing regions constituting the C-terminus of Spt5, play a redundant role in repressing RNAPII elongation in vivo, overview. Mutant NSpt5, lacking the C-terminus, directly associates with hsp70-4 chromatin in vivo and increases the occupancy of RNAPII, positive transcription elongation factor b, histone H3 Lys 4 trimethylation, and surprisingly, the negative elongation factor A at the locus, indicating a direct action of NSpt5 on the elongation repressed locus, nuclear extracts containing the constitutively active P-TEFb and WT DSIF lead to a time-dependent increase of the long, promoter-distal RNase T1-resistant products, reflecting the elongation stimulatory activity of Spt5, overview Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.6 nucleus
-
 Danio rerio 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn Danio rerio transcription elongation as a critical regulatory step in addition to initiation diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.6 Danio rerio
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.6 nucleoside triphosphate + RNAn
-
 Danio rerio diphosphate + RNAn+1
-
 ?
2.7.7.6 nucleoside triphosphate + RNAn transcription elongation as a critical regulatory step in addition to initiation Danio rerio diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.6 RNA polymerase II
-
 Danio rerio
2.7.7.6 RNAPII
-
 Danio rerio