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Literature summary extracted from

  • Del Viso, F.; Puebla, A.; Hopp, H.; Heinz, R.
    Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in the cold tolerant Patagonian species Bromus pictus (2009), Planta, 231, 13-25.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.153 expressed in Pichia pastoris Bromus pictus
3.2.1.153 single copy gene Bp1-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional expression in Pichia pastoris Bromus pictus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.153 additional information about 50% decreased enzyme activity in the first leaf section after 7 days of cold treatment, fructan accumulation profile along with cold treatment, overview Bromus pictus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.153 additional information Bromus pictus Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.153 Bromus pictus
-
 a cold tolerant, graminean decaploid species from the Patagonian region of Argentina, gene Bp1-FEHa
-
3.2.1.153 Bromus pictus D2IGW7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.153 leaf
-
 Bromus pictus
-
3.2.1.153 leaf meature leaves, highest Bp1-FEHa expression levels in the second section of the sheath and the tip of the blade Bromus pictus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.153 1-kestotriose + H2O 1-FEHa is a fructan exohydrolase with predominant activity towards beta-(2-1) linkages Bromus pictus ?
-
 ?
3.2.1.153 6-kestotriose + H2O lower activity compared to 1-kestotriose Bromus pictus ?
-
 ?
3.2.1.153 additional information Bp1-FEHa is a fructan exohydrolase with predominant activity towards beta-2,1-linkages Bromus pictus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.153 1-FEH
-
 Bromus pictus
3.2.1.153 1-FEHa
-
 Bromus pictus
3.2.1.153 fructan 1-exohydrolase
-
 Bromus pictus