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Literature summary extracted from

  • Ding, H.; Zhang, A.; Wang, J.; Lu, R.; Zhang, H.; Zhang, J.; Jiang, M.
    Identity of an ABA-activated 46 kDa mitogen-activated protein kinase from Zea mays leaves: partial purification, identification and characterization (2009), Planta, 230, 239-251.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.24 abscisic acid ABA Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.24 PD98059
-
 Zea mays
2.7.11.24 U0126
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.24 additional information
-
 additional information apparent Km for myelin basic protein is 0.13 microg microl-1 Zea mays
2.7.11.24 0.062
-
 ATP apparent KM Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.24 Mg2+ 10 mM MgCl2 Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.24 45000
-
 determined by SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.24 ATP + a protein Zea mays
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.24 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.24 partially purified from maize by Q-Sepharose FF, Phenyl-Sepharose FF, Resource Q, Mono QTM 5/50 GL, poly-L-lysine agarose, and Superdex 75 prep-grade columns Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.24 0.000002
-
 homogenate Zea mays
2.7.11.24 0.000017
-
 purification step (NH4)2SO4 30% Zea mays
2.7.11.24 0.00002
-
 purification step centrifugation 100000 X g Zea mays
2.7.11.24 0.000085
-
 purification step Q-Sepharose FF Zea mays
2.7.11.24 0.00024
-
 purification step Phenyl-Sepharose FF Zea mays
2.7.11.24 0.000834
-
 purification step Resource Q Zea mays
2.7.11.24 0.001804
-
 purification step Mono Q 5/50 GL Zea mays
2.7.11.24 0.009005
-
 purification step poly-L-lysine-agarose Zea mays
2.7.11.24 0.02042
-
 purification step HiLoad 16/60 Superdex 75 pg Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.24 ATP + a protein
-
 Zea mays ADP + a phosphoprotein
-
 ?
2.7.11.24 ATP + myelin basic protein
-
 Zea mays ADP + phosphorylated myelin basic protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.24 monomer
-
 Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.11.24 MAP kinase
-
 Zea mays
2.7.11.24 mitogen-activated kinase
-
 Zea mays
2.7.11.24 mitogen-activated protein kinase
-
 Zea mays
2.7.11.24 p46MAPK
-
 Zea mays
2.7.11.24 ZmMAPK5
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.24 22
-
 in-gel kinase activity assay at room temperature Zea mays
2.7.11.24 30
-
-
 Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.11.24
-
 70
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.24 7.5
-
 in-gel kinase activity assay Zea mays
2.7.11.24 8
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.24 5 10
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.24 ATP
-
 Zea mays