EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.123 | coding region of BhGolS1 is inframe cloned into pGEX-4T-1 downstream to GST coding region and transformed into Escherichia coli BL21(DE3) and BL21 codon plus cells, generation of transgenic tobacco plants, constructs are introduced into Agrobacterium strain LBA4404 by electroporation and transformed into tobacco via a leaf disc method | Dorcoceras hygrometricum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.123 | MnCl2 | 5 mM are included in assay medium | Dorcoceras hygrometricum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.123 | UDP-galactose + myo-inositol | Dorcoceras hygrometricum | - |
UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol | i.e. galactinol | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.123 | Dorcoceras hygrometricum | B6UYJ2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.123 | the soluble protein is separated by SDS-PAGE to check the molecular weight of the recombinant protein, which is then purified using Glutathione Sepharose TM 4B | Dorcoceras hygrometricum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.123 | leaf | - |
Dorcoceras hygrometricum | - |
2.4.1.123 | seedling | - |
Dorcoceras hygrometricum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.123 | additional information | - |
BhGolS1 activity enhances drought tolerance in transgenic tobacco, a higher survival rate of transgenic plants after rewatering (84.8% for S10-1 tobacco line, 72.6% for S22-5 tobacco line, 61.5% for Rd15-6 tobacco line), compared to wild type (38.4%) is observed | Dorcoceras hygrometricum |
2.4.1.123 | 3.8 | - |
purified BhGolS1-GST fusion protein is able to catalyze galactinol synthesis, 50 mM Hepes buffer, pH 7.0, 2 mM dithiothreitol, 5 mM MnCl2 and 0.5-1 mg purified enzyme protein | Dorcoceras hygrometricum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.123 | UDP-galactose + myo-inositol | - |
Dorcoceras hygrometricum | UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol | i.e. galactinol | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.123 | BhGolS1 | - |
Dorcoceras hygrometricum |
2.4.1.123 | galactinol synthase | - |
Dorcoceras hygrometricum |
2.4.1.123 | GOLS | - |
Dorcoceras hygrometricum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.123 | 30 | - |
assay at | Dorcoceras hygrometricum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.123 | 7 | - |
assay at | Dorcoceras hygrometricum |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.4.1.123 | Dorcoceras hygrometricum | a WRKY transcription factor participates in dehydration tolerance in Boea hygrometrica by binding to the W-box elements of the galactinol synthase promoter, GolS genes are induced by a variety of stresses in both stress-sensitive and tolerant-plant species, mRNA and protein accumulate in leaves dehydrated for 2-48 h, and disappear after rehydration, BhGolS1 is rapidly induced by abscisic acid at the mRNA level after 0.5 h and at the protein level after 8 h | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.1.123 | physiological function | first committed step of the raffinose family oligosaccharides biosynthetic pathway | Dorcoceras hygrometricum |