Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mühlenweg, A.; Melzer, M.; Li, S.M.; Heide, L.
    4-Hydroxybenzoate 3-geranyltransferase from Lithospermum erythrorhizon: purification of a plant membrane-bound prenyltransferase (1998), Planta, 205, 407-413.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.93 4-chloro-mercuriphenylsulfonic acid 0.1 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon
2.5.1.93 Digitonin
-
 Lithospermum erythrorhizon
2.5.1.93 iodoacetamide 10 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon
2.5.1.93 N-Methylmaleimide 10 mM, completely inhibits 4HB geranyltransferase activity Lithospermum erythrorhizon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.93 0.0138
-
 geranyl diphosphate pH 7.8, 37°C Lithospermum erythrorhizon
2.5.1.93 0.0184
-
 4-hydroxybenzoate pH 7.8, 37°C Lithospermum erythrorhizon

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.93 Mg2+ required, optimal concentration: 20-50 mM Lithospermum erythrorhizon

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.93 341000
-
 gel filtration Lithospermum erythrorhizon

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.93 geranyl diphosphate + 4-hydroxybenzoate Lithospermum erythrorhizon regulatory enzyme in the biosynthesis of shikonin 3-geranyl-4-hydroxybenzoate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.93 Lithospermum erythrorhizon
-
 Sieb. et Zucc.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.93 Of various detergents examined, digitonin is the most suitable for the solubilization of the enzyme Lithospermum erythrorhizon

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.93 cell suspension culture
-
 Lithospermum erythrorhizon
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.93 1.32
-
-
 Lithospermum erythrorhizon

Storage Stability

EC Number Storage Stability Organism
2.5.1.93 -20°C, in the presence of 10% glycerol, frozen enzyme preparations are stable upon storage for at least two months. Protease inhibitors directed against serin-, carboxyl-, and metalloproteases such as PMSF, 1,2-epoxy-3-(4-nitrophenoxy)-propane or EGTA do not increase the stability of the enzyme solution. Lithospermum erythrorhizon

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.93 geranyl diphosphate + 4-hydroxybenzoate regulatory enzyme in the biosynthesis of shikonin Lithospermum erythrorhizon 3-geranyl-4-hydroxybenzoate + diphosphate
-
 ?
2.5.1.93 geranyl diphosphate + 4-hydroxybenzoate the enzyme is highly specific for geranyl diphosphate and 4-hydroxybenzoate Lithospermum erythrorhizon 3-geranyl-4-hydroxybenzoate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.93 4HB geranyltransferase
-
 Lithospermum erythrorhizon

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.93 46
-
-
 Lithospermum erythrorhizon

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.93 35 63 half-maximal activity at 35°C and at 63°C Lithospermum erythrorhizon

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.93 7.7
-
 in Bis-Tris-HCl buffer Lithospermum erythrorhizon
2.5.1.93 8.5
-
 in Tris-HCl buffer Lithospermum erythrorhizon

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.93 6.2 9.3 half-maximal activity at pH 6.2 and at pH 9.3, Tris-HCl buffer Lithospermum erythrorhizon

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.5.1.93 4
-
 at pH 4 or 8, all activtiy is lost quickly Lithospermum erythrorhizon
2.5.1.93 5
-
 storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days Lithospermum erythrorhizon
2.5.1.93 6
-
 at pH 6, the enzyme can be stored in the presence of 1.5 mM digitonin, 10% glycerol and 2 mM DTT at 4°C for two weeks with minimal loss of activity Lithospermum erythrorhizon
2.5.1.93 7
-
 storage at pH 5 or pH 7 results in a loss of activity between 20% and 30% in 8 days Lithospermum erythrorhizon
2.5.1.93 8
-
 at pH 4 or 8, all activtiy is lost quickly Lithospermum erythrorhizon

General Information

EC Number General Information Comment Organism
2.5.1.93 physiological function regulatory enzyme in the biosynthesis of shikonin Lithospermum erythrorhizon