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Literature summary extracted from

  • Maisonneuve, S.; Bessoule, J.J.; Lessire, R.; Delseny, M.; Roscoe, T.J.
    Expression of Brassica napus microsomal Lysophosphatidic Acid Acyltransferase isozymes enhances seed oil content in Arabidopsis thaliana (2009), Plant Physiol., 152, 670-684.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.51 genes BAT1.13 and BAT1.5, cloning of two cDNAs encoding microsomal isozymes from an immature embryo library, sequence comparisons, functional expression of the two rapeseed microsomal LPAAT isozymes in Arabidopsis thaliana, using Agrobacterium tumefaciens strain C58C1 transfection, leading to enhanced oil content and seed weight, tissue localization study, overview. Complementationof a thermosensitive Escherichia coli strain JC201 by expression of Bat1.5 and BAT1.13, overview Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.51 microsome LPAAT is located in the cytoplasmic endomembrane compartment, two microsomal LPAAT isozymes are identified. Diversity of LPAAT microsomal isozymes in rapeseed, overview Brassica napus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.51 Mg2+
-
 Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Brassica napus i.e. lysophosphatidic acid CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.51 Brassica napus Q9XFW4 BAT1.13 and BAT1.5 genes encoding two microsomal LPAAT isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.51 flower BAT1.13 Brassica napus
-
2.3.1.51 leaf BAT1.13 Brassica napus
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2.3.1.51 additional information differences in the expression of the two genes emcoding the two microsomal LPAAT isozymes, one is detected in all rapeseed tissues and during silique and seed development, whereas the expression of the second gene is restricted predominantly to siliques and developing seeds Brassica napus
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2.3.1.51 root BAT1.13, expression at the tip of primary and secondary roots and in mesophyll/cortex tissue behind the elongation zone and in the vascular tissue Brassica napus
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2.3.1.51 seed developing, BAT1.5 , and BAT1.13 Brassica napus
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2.3.1.51 silique young, BAT1.5 , and BAT1.13 Brassica napus
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2.3.1.51 stem BAT1.13 Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.51 acyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Brassica napus CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
2.3.1.51 oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate i.e. lysophosphatidic acid Brassica napus CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.51 LPAAT
-
 Brassica napus
2.3.1.51 lysophosphatidic acid acyltransferase
-
 Brassica napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.51 30
-
 assay at Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.51 8
-
 assay at Brassica napus

General Information

EC Number General Information Comment Organism
2.3.1.51 metabolism LPAAT is a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds Brassica napus
2.3.1.51 physiological function LPAAT plays an essential role in the synthesis of phosphatidic acid Brassica napus