BRENDA - Enzyme Database

Phosphoenolpyruvate carboxylase from C4 leaves is selectively targeted for inhibition by anionic phospholipids

Monreal, J.; McLoughlin, F.; Echevarría, C.; García-Mauriño, S.; Testerink, C.; Plant Physiol. 152, 634-638 (2010)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
lyso-phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 45% of the control activity
Sorghum bicolor
4.1.1.31
lyso-phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 45% of the control activity
Zea mays
4.1.1.31
additional information
not inhibited by phosphatidic acid, phosphatidylinositol, phosphatidylinositol 4-phosphate, lyso-phosphatidic acid, phosphatidylserine, phosphatidylcholine, and phosphatidylethanolamine
Arabidopsis thaliana
4.1.1.31
additional information
phosphatidylcholine and phosphatidylethanolamine have no effect on enzyme activity
Sorghum bicolor
4.1.1.31
additional information
phosphatidylcholine and phosphatidylethanolamine have no effect on enzyme activity
Zea mays
4.1.1.31
phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity. Inclusion of D-glucose 6-phosphate or L-malate do not change the effect of phosphatidic acid on PEPC, preincubation of the enzyme with 5 mM phosphoenolpyruvate prior to the addition of phosphatidic acid did not prevent inactivation either. The incubation of phosphatidic acid-inactivated PEPC with protein kinase A does not restore PEPC activity
Sorghum bicolor
4.1.1.31
phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity. Inclusion of D-glucose 6-phosphate or L-malate do not change the effect of phosphatidic acid on PEPC, preincubation of the enzyme with 5 mM phosphoenolpyruvate prior to the addition of phosphatidic acid did not prevent inactivation either. The incubation of phosphatidic acid-inactivated PEPC with protein kinase A does not restore PEPC activity
Zea mays
4.1.1.31
phosphatidylinositol
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylinositol
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity
Zea mays
4.1.1.31
phosphatidylinositol 4-phosphate
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 50% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylinositol 4-phosphate
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 50% of the control activity
Zea mays
4.1.1.31
phosphatidylserine
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 80% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylserine
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 80% of the control activity
Zea mays
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.31
membrane
the enzyme partially localizes to membranes
Arabidopsis thaliana
16020
-
4.1.1.31
membrane
the enzyme partially localizes to membranes
Sorghum bicolor
16020
-
4.1.1.31
membrane
the enzyme partially localizes to membranes
Zea mays
16020
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.31
Arabidopsis thaliana
-
-
-
4.1.1.31
Sorghum bicolor
-
-
-
4.1.1.31
Zea mays
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.31
leaf
-
Arabidopsis thaliana
-
4.1.1.31
leaf
-
Sorghum bicolor
-
4.1.1.31
leaf
-
Zea mays
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Zea mays
phosphate + oxaloacetate
-
-
-
ir
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Arabidopsis thaliana
phosphate + oxaloacetate
-
-
-
ir
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Sorghum bicolor
phosphate + oxaloacetate
-
-
-
ir
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.31
lyso-phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 45% of the control activity
Sorghum bicolor
4.1.1.31
lyso-phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 45% of the control activity
Zea mays
4.1.1.31
additional information
not inhibited by phosphatidic acid, phosphatidylinositol, phosphatidylinositol 4-phosphate, lyso-phosphatidic acid, phosphatidylserine, phosphatidylcholine, and phosphatidylethanolamine
Arabidopsis thaliana
4.1.1.31
additional information
phosphatidylcholine and phosphatidylethanolamine have no effect on enzyme activity
Sorghum bicolor
4.1.1.31
additional information
phosphatidylcholine and phosphatidylethanolamine have no effect on enzyme activity
Zea mays
4.1.1.31
phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity. Inclusion of D-glucose 6-phosphate or L-malate do not change the effect of phosphatidic acid on PEPC, preincubation of the enzyme with 5 mM phosphoenolpyruvate prior to the addition of phosphatidic acid did not prevent inactivation either. The incubation of phosphatidic acid-inactivated PEPC with protein kinase A does not restore PEPC activity
Sorghum bicolor
4.1.1.31
phosphatidic acid
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity. Inclusion of D-glucose 6-phosphate or L-malate do not change the effect of phosphatidic acid on PEPC, preincubation of the enzyme with 5 mM phosphoenolpyruvate prior to the addition of phosphatidic acid did not prevent inactivation either. The incubation of phosphatidic acid-inactivated PEPC with protein kinase A does not restore PEPC activity
Zea mays
4.1.1.31
phosphatidylinositol
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylinositol
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 40% of the control activity
Zea mays
4.1.1.31
phosphatidylinositol 4-phosphate
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 50% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylinositol 4-phosphate
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 50% of the control activity
Zea mays
4.1.1.31
phosphatidylserine
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 80% of the control activity
Sorghum bicolor
4.1.1.31
phosphatidylserine
addition of 0.05 mM phosphatidic acid decreases PEPC activity to approximately 80% of the control activity
Zea mays
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.1.1.31
membrane
the enzyme partially localizes to membranes
Arabidopsis thaliana
16020
-
4.1.1.31
membrane
the enzyme partially localizes to membranes
Sorghum bicolor
16020
-
4.1.1.31
membrane
the enzyme partially localizes to membranes
Zea mays
16020
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.1.31
leaf
-
Arabidopsis thaliana
-
4.1.1.31
leaf
-
Sorghum bicolor
-
4.1.1.31
leaf
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Zea mays
phosphate + oxaloacetate
-
-
-
ir
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Arabidopsis thaliana
phosphate + oxaloacetate
-
-
-
ir
4.1.1.31
phosphoenolpyruvate + CO2
-
706331
Sorghum bicolor
phosphate + oxaloacetate
-
-
-
ir