BRENDA - Enzyme Database show

CYP704B1 is a long-chain fatty acid omega-hydroxylase essential for sporopollenin synthesis in pollen of Arabidopsis

Dobritsa, A.A.; Shrestha, J.; Morant, M.; Pinot, F.; Matsuno, M.; Swanson, R.; Moller, B.L.; Preuss, D.; Plant Physiol. 151, 574-589 (2009)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.80
expression in yeast WAT11 cells
Arabidopsis thaliana
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.80
Arabidopsis thaliana
Q9C788
isoform CYP704B1
-
1.14.14.130
Arabidopsis thaliana
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.80
anther
CYP704B1 is expressed in the developing anthers
Arabidopsis thaliana
-
1.14.14.130
anther
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.80
9,10-epoxystearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxy-9,10-epoxystearic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
best substrate
706321
Arabidopsis thaliana
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
additional information
no substrate: 12-hydroxylauric acid, lauric acid, cinnamate, p-coumarate, ferulate, sinapate, and p-coumaroyl shikimate
706321
Arabidopsis thaliana
?
-
-
-
-
1.14.14.80
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
28% yield
706321
Arabidopsis thaliana
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
21% yield
706321
Arabidopsis thaliana
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
44% yield
706321
Arabidopsis thaliana
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.130
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
12-hydroxydodecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.80
expression in yeast WAT11 cells
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.80
anther
CYP704B1 is expressed in the developing anthers
Arabidopsis thaliana
-
1.14.14.130
anther
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.80
9,10-epoxystearic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxy-9,10-epoxystearic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
linoleic acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
18-hydroxylinoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
linolenic acid + [reduced NADPH-hemoprotein reductase] + O2
best substrate
706321
Arabidopsis thaliana
18-hydroxylinolenic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
additional information
no substrate: 12-hydroxylauric acid, lauric acid, cinnamate, p-coumarate, ferulate, sinapate, and p-coumaroyl shikimate
706321
Arabidopsis thaliana
?
-
-
-
-
1.14.14.80
oleic acid + [reduced NADPH-hemoprotein reductase] + O2
28% yield
706321
Arabidopsis thaliana
18-hydroxyoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
palmitic acid + [reduced NADPH-hemoprotein reductase] + O2
21% yield
706321
Arabidopsis thaliana
16-hydroxypalmitic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.80
palmitoleic acid + [reduced NADPH-hemoprotein reductase] + O2
44% yield
706321
Arabidopsis thaliana
18-hydroxypalmitoleic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.130
lauric acid + [reduced NADPH-hemoprotein reductase] + O2
-
706321
Arabidopsis thaliana
12-hydroxydodecanoic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.14.80
Arabidopsis thaliana
CYP704B1 is highly coexpressed with CYP703A2, a cytochrome P450 involved in exine production
additional information
General Information
EC Number
General Information
Commentary
Organism
1.14.14.80
physiological function
mutations in CYP704B1 result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebra phenotyp. Mutations in three genes, CYP704B1, CYP703A2, and another exine gene, MALE STERILITY2 result in pollen with remarkably similar zebra phenotypes, distinct from those of other known exine mutants. The double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
1.14.15.3
malfunction
mutations in CYP703A2 or CYP704B1 genes result in pollen with remarkably similar zebra phenotypes (impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface). Double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
1.14.15.3
physiological function
CYP703A2 and CYP704B1 together with the fatty acyl reductase MALE STERILITY2 are required to provide an indispensable subset of fatty acid-derived components within the sporopollenin biosynthesis framework
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.80
physiological function
mutations in CYP704B1 result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebra phenotyp. Mutations in three genes, CYP704B1, CYP703A2, and another exine gene, MALE STERILITY2 result in pollen with remarkably similar zebra phenotypes, distinct from those of other known exine mutants. The double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
1.14.15.3
malfunction
mutations in CYP703A2 or CYP704B1 genes result in pollen with remarkably similar zebra phenotypes (impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface). Double and triple mutant combinations do not result in the appearance of novel phenotypes or enhancement of single mutant phenotypes
Arabidopsis thaliana
1.14.15.3
physiological function
CYP703A2 and CYP704B1 together with the fatty acyl reductase MALE STERILITY2 are required to provide an indispensable subset of fatty acid-derived components within the sporopollenin biosynthesis framework
Arabidopsis thaliana
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.14.80
Arabidopsis thaliana
CYP704B1 is highly coexpressed with CYP703A2, a cytochrome P450 involved in exine production
additional information