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Literature summary extracted from

  • Kuang, R.; Chan, K.H.; Yeung, E.; Lim, B.L.
    Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis (2009), Plant Physiol., 151, 199-209.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.2 expression in Nicotiana tabacum Arabidopsis thaliana
3.1.3.2 His-tagged version expressed inNicotiana tabacum, GST-fusion protein (without any enzyme activity) expressed in Escherichia coli Arabidopsis thaliana
3.1.3.8 expression in Nicotiana tabacum Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 Cu2+ 17.8% of initial activity; inhibites phytase activity Arabidopsis thaliana
3.1.3.2 F- reduces phytase activity Arabidopsis thaliana
3.1.3.2 molybdate 0.25 mM: enzymatic activity completely abolished Arabidopsis thaliana
3.1.3.2 additional information neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3- Arabidopsis thaliana
3.1.3.2 PO43- reduces phytase activity Arabidopsis thaliana
3.1.3.2 SO3- 43.1% of initial activity Arabidopsis thaliana
3.1.3.2 SO32- inhibites phytase activity Arabidopsis thaliana
3.1.3.8 Cu2+ 17.8% of initial activity Arabidopsis thaliana
3.1.3.8 additional information neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3- Arabidopsis thaliana
3.1.3.8 SO3- 43.1% of initial activity Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 0.278
-
sodium phytate
-
Arabidopsis thaliana
3.1.3.2 0.278
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 0.703
-
p-nitrophenyl phosphate
-
Arabidopsis thaliana
3.1.3.2 0.703
-
4-nitrophenyl phosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 0.801
-
phosphoenolpyruvate
-
Arabidopsis thaliana
3.1.3.2 0.801
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 0.278
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 0.703
-
4-nitrophenylphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 0.801
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Ca2+ stimulates phytase activity Arabidopsis thaliana
3.1.3.2 Ca2+ 127% of initial activity Arabidopsis thaliana
3.1.3.2 Zn2+ stimulates phytase activity Arabidopsis thaliana
3.1.3.2 Zn2+ 129.4%, of initial activity Arabidopsis thaliana
3.1.3.8 Ca2+ 127% of initial activity Arabidopsis thaliana
3.1.3.8 Zn2+ 129.4%, of initial activity Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.2 58000
-
gel filtration Arabidopsis thaliana
3.1.3.2 60000
-
His-tagged protein, SDS-PAGE, gel filtration Arabidopsis thaliana
3.1.3.2 60000
-
1 * 60000, SDS-PAGE Arabidopsis thaliana
3.1.3.8 58000
-
gel filtration Arabidopsis thaliana
3.1.3.8 60000
-
1 * 60000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Arabidopsis thaliana
-
purple acid phosphatase with phytase activity
-
3.1.3.2 Arabidopsis thaliana Q9SFU3
-
-
3.1.3.8 Arabidopsis thaliana
-
purple acid phosphatase with phytase activity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 cation-exchange chromatography, Ni2+ affinity chromatography, gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.2 pollen strong expression in the early stages of seedling growth and pollen germination Arabidopsis thaliana
-
3.1.3.2 root
-
Arabidopsis thaliana
-
3.1.3.2 seedling strong expression in the early stages of seedling growth and pollen germination Arabidopsis thaliana
-
3.1.3.2 vasculature
-
Arabidopsis thaliana
-
3.1.3.8 pollen strong expression in the early stages of seedling growth and pollen germination Arabidopsis thaliana
-
3.1.3.8 root
-
Arabidopsis thaliana
-
3.1.3.8 seedling strong expression in the early stages of seedling growth and pollen germination Arabidopsis thaliana
-
3.1.3.8 vasculature
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 10
-
37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 13.4
-
substrate: sodium phytate Arabidopsis thaliana
3.1.3.2 134.7
-
substrate: phosphoenolpyruvate Arabidopsis thaliana
3.1.3.2 165.4
-
substrate: p-nitrophenyl phosphate Arabidopsis thaliana
3.1.3.8 10
-
37°C, pH 4.5 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 3-phosphoglycerate + H2O 69% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerate + phosphate
-
?
3.1.3.2 4-nitrophenyl phosphate + H2O
-
Arabidopsis thaliana 4-nitrophenol + phosphate
-
?
3.1.3.2 ADP + H2O 50% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 D-glucose 6-phosphate + H2O 20% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana D-glucose + phosphate
-
?
3.1.3.2 dATP + H2O 31% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 dCTP + H2O 35% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 dGTP + H2O 52% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 diphosphate + H2O
-
Arabidopsis thaliana 2 phosphate
-
?
3.1.3.2 diphosphate + H2O 95% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana 2 phosphate
-
?
3.1.3.2 dTTP + H2O 37% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 glycerol 3-phosphate + H2O 43% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerol + phosphate
-
?
3.1.3.2 myo-inositol hexakisphosphate + H2O 7% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.2 O-phospho-L-serine + H2O 25% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-serine + phosphate
-
?
3.1.3.2 O-phospho-L-tyrosine + H2O 28% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-tyrosine + phosphate
-
?
3.1.3.2 p-nitrophenyl phosphate + H2O further substrates: 3-phosphoglyceric acid, ADP, dGTP, glucose 3-phosphate, dTTP, dCTP, dATP, O-phospho-Tyr, O-phospho-Ser, glucose 6-phosphate, CMP, O-phospho-Thr, ribose 5-phosphate, ATP, fructose 6-phosphate, AMP, GMP Arabidopsis thaliana p-nitrophenol + phosphate
-
?
3.1.3.2 phosphoenolpyruvate + H2O
-
Arabidopsis thaliana pyruvate + phosphate
-
?
3.1.3.2 phosphoenolpyruvate + H2O 92% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana pyruvate + phosphate
-
?
3.1.3.2 phytate + H2O
-
Arabidopsis thaliana ? + phosphate
-
?
3.1.3.2 sodium phytate + H2O
-
Arabidopsis thaliana ?
-
?
3.1.3.8 3-phosphoglycerate + H2O 69% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerate + phosphate
-
?
3.1.3.8 4-nitrophenylphosphate + H2O
-
Arabidopsis thaliana 4-nitrophenol + phosphate
-
?
3.1.3.8 ADP + H2O 50% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.8 D-glucose 6-phosphate + H2O 20% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana D-glucose + phosphate
-
?
3.1.3.8 dATP + H2O 31% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.8 dCTP + H2O 35% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.8 dGTP + H2O 52% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.8 diphosphate + H2O 95% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana 2 phosphate
-
?
3.1.3.8 dTTP + H2O 37% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana ? + phosphate
-
?
3.1.3.8 glycerol 3-phosphate + H2O 43% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana glycerol + phosphate
-
?
3.1.3.8 myo-inositol hexakisphosphate + H2O 7% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
?
3.1.3.8 O-phospho-L-serine + H2O 25% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-serine + phosphate
-
?
3.1.3.8 O-phospho-L-tyrosine + H2O 28% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana L-tyrosine + phosphate
-
?
3.1.3.8 phosphoenolpyruvate + H2O 92% of the activity with 4-nitrophenylphosphate Arabidopsis thaliana pyruvate + phosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.2 monomer 1 * 60000, SDS-PAGE Arabidopsis thaliana
3.1.3.2 monomer 1 * 60000, His-tagged protein, SDS-PAGE, gel filtration Arabidopsis thaliana
3.1.3.8 monomer 1 * 60000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.1.3.2 AtPAP15
-
Arabidopsis thaliana
3.1.3.2 purple acid phosphatase
-
Arabidopsis thaliana
3.1.3.8 AtPAP15
-
Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.2 23 37
-
Arabidopsis thaliana
3.1.3.2 23 37 highest activity Arabidopsis thaliana
3.1.3.8 23 37
-
Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.2 23 37 most stable Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.2 8.85
-
sodium phytate
-
Arabidopsis thaliana
3.1.3.2 8.85
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 161.9
-
p-nitrophenyl phosphate
-
Arabidopsis thaliana
3.1.3.2 161.9
-
4-nitrophenyl phosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 198
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 198.6
-
phosphoenolpyruvate
-
Arabidopsis thaliana
3.1.3.8 8.85
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 161.9
-
4-nitrophenylphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 198
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 4.5
-
-
Arabidopsis thaliana
3.1.3.8 4.5
-
-
Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
3.1.3.2 Arabidopsis thaliana expressed in the vasculature, pollen grains and roots additional information

General Information

EC Number General Information Comment Organism
3.1.3.2 physiological function gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate Arabidopsis thaliana
3.1.3.8 physiological function gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.3.2 26.7
-
sodium phytate
-
Arabidopsis thaliana
3.1.3.2 31.8
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 232
-
4-nitrophenyl phosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 241.3
-
p-nitrophenyl phosphate
-
Arabidopsis thaliana
3.1.3.2 248
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.2 253.8
-
phosphoenolpyruvate
-
Arabidopsis thaliana
3.1.3.8 31.8
-
myo-inositol hexakisphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 232
-
4-nitrophenylphosphate 37°C, pH 4.5 Arabidopsis thaliana
3.1.3.8 248
-
phosphoenolpyruvate 37°C, pH 4.5 Arabidopsis thaliana