EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.2 | expression in Nicotiana tabacum | Arabidopsis thaliana |
3.1.3.2 | His-tagged version expressed inNicotiana tabacum, GST-fusion protein (without any enzyme activity) expressed in Escherichia coli | Arabidopsis thaliana |
3.1.3.8 | expression in Nicotiana tabacum | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Cu2+ | 17.8% of initial activity; inhibites phytase activity | Arabidopsis thaliana | |
3.1.3.2 | F- | reduces phytase activity | Arabidopsis thaliana | |
3.1.3.2 | molybdate | 0.25 mM: enzymatic activity completely abolished | Arabidopsis thaliana | |
3.1.3.2 | additional information | neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3- | Arabidopsis thaliana | |
3.1.3.2 | PO43- | reduces phytase activity | Arabidopsis thaliana | |
3.1.3.2 | SO3- | 43.1% of initial activity | Arabidopsis thaliana | |
3.1.3.2 | SO32- | inhibites phytase activity | Arabidopsis thaliana | |
3.1.3.8 | Cu2+ | 17.8% of initial activity | Arabidopsis thaliana | |
3.1.3.8 | additional information | neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3- | Arabidopsis thaliana | |
3.1.3.8 | SO3- | 43.1% of initial activity | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 0.278 | - |
sodium phytate | - |
Arabidopsis thaliana | |
3.1.3.2 | 0.278 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 0.703 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
3.1.3.2 | 0.703 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 0.801 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
3.1.3.2 | 0.801 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 0.278 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 0.703 | - |
4-nitrophenylphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 0.801 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.2 | Ca2+ | stimulates phytase activity | Arabidopsis thaliana | |
3.1.3.2 | Ca2+ | 127% of initial activity | Arabidopsis thaliana | |
3.1.3.2 | Zn2+ | stimulates phytase activity | Arabidopsis thaliana | |
3.1.3.2 | Zn2+ | 129.4%, of initial activity | Arabidopsis thaliana | |
3.1.3.8 | Ca2+ | 127% of initial activity | Arabidopsis thaliana | |
3.1.3.8 | Zn2+ | 129.4%, of initial activity | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 58000 | - |
gel filtration | Arabidopsis thaliana |
3.1.3.2 | 60000 | - |
His-tagged protein, SDS-PAGE, gel filtration | Arabidopsis thaliana |
3.1.3.2 | 60000 | - |
1 * 60000, SDS-PAGE | Arabidopsis thaliana |
3.1.3.8 | 58000 | - |
gel filtration | Arabidopsis thaliana |
3.1.3.8 | 60000 | - |
1 * 60000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.2 | Arabidopsis thaliana | - |
purple acid phosphatase with phytase activity | - |
3.1.3.2 | Arabidopsis thaliana | Q9SFU3 | - |
- |
3.1.3.8 | Arabidopsis thaliana | - |
purple acid phosphatase with phytase activity | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.2 | cation-exchange chromatography, Ni2+ affinity chromatography, gel filtration | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.2 | pollen | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
3.1.3.2 | root | - |
Arabidopsis thaliana | - |
3.1.3.2 | seedling | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
3.1.3.2 | vasculature | - |
Arabidopsis thaliana | - |
3.1.3.8 | pollen | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
3.1.3.8 | root | - |
Arabidopsis thaliana | - |
3.1.3.8 | seedling | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
3.1.3.8 | vasculature | - |
Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 10 | - |
37°C, pH 4.5 | Arabidopsis thaliana |
3.1.3.2 | 13.4 | - |
substrate: sodium phytate | Arabidopsis thaliana |
3.1.3.2 | 134.7 | - |
substrate: phosphoenolpyruvate | Arabidopsis thaliana |
3.1.3.2 | 165.4 | - |
substrate: p-nitrophenyl phosphate | Arabidopsis thaliana |
3.1.3.8 | 10 | - |
37°C, pH 4.5 | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.2 | 3-phosphoglycerate + H2O | 69% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerate + phosphate | - |
? | |
3.1.3.2 | 4-nitrophenyl phosphate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
3.1.3.2 | ADP + H2O | 50% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | D-glucose 6-phosphate + H2O | 20% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | D-glucose + phosphate | - |
? | |
3.1.3.2 | dATP + H2O | 31% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | dCTP + H2O | 35% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | dGTP + H2O | 52% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | diphosphate + H2O | - |
Arabidopsis thaliana | 2 phosphate | - |
? | |
3.1.3.2 | diphosphate + H2O | 95% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | 2 phosphate | - |
? | |
3.1.3.2 | dTTP + H2O | 37% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | glycerol 3-phosphate + H2O | 43% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
3.1.3.2 | myo-inositol hexakisphosphate + H2O | 7% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
3.1.3.2 | O-phospho-L-serine + H2O | 25% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-serine + phosphate | - |
? | |
3.1.3.2 | O-phospho-L-tyrosine + H2O | 28% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-tyrosine + phosphate | - |
? | |
3.1.3.2 | p-nitrophenyl phosphate + H2O | further substrates: 3-phosphoglyceric acid, ADP, dGTP, glucose 3-phosphate, dTTP, dCTP, dATP, O-phospho-Tyr, O-phospho-Ser, glucose 6-phosphate, CMP, O-phospho-Thr, ribose 5-phosphate, ATP, fructose 6-phosphate, AMP, GMP | Arabidopsis thaliana | p-nitrophenol + phosphate | - |
? | |
3.1.3.2 | phosphoenolpyruvate + H2O | - |
Arabidopsis thaliana | pyruvate + phosphate | - |
? | |
3.1.3.2 | phosphoenolpyruvate + H2O | 92% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | pyruvate + phosphate | - |
? | |
3.1.3.2 | phytate + H2O | - |
Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.2 | sodium phytate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
3.1.3.8 | 3-phosphoglycerate + H2O | 69% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerate + phosphate | - |
? | |
3.1.3.8 | 4-nitrophenylphosphate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
3.1.3.8 | ADP + H2O | 50% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.8 | D-glucose 6-phosphate + H2O | 20% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | D-glucose + phosphate | - |
? | |
3.1.3.8 | dATP + H2O | 31% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.8 | dCTP + H2O | 35% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.8 | dGTP + H2O | 52% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.8 | diphosphate + H2O | 95% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | 2 phosphate | - |
? | |
3.1.3.8 | dTTP + H2O | 37% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
3.1.3.8 | glycerol 3-phosphate + H2O | 43% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
3.1.3.8 | myo-inositol hexakisphosphate + H2O | 7% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | myo-inositol 1,2,4,5,6-pentakisphosphate + myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
3.1.3.8 | O-phospho-L-serine + H2O | 25% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-serine + phosphate | - |
? | |
3.1.3.8 | O-phospho-L-tyrosine + H2O | 28% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-tyrosine + phosphate | - |
? | |
3.1.3.8 | phosphoenolpyruvate + H2O | 92% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | pyruvate + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.2 | monomer | 1 * 60000, SDS-PAGE | Arabidopsis thaliana |
3.1.3.2 | monomer | 1 * 60000, His-tagged protein, SDS-PAGE, gel filtration | Arabidopsis thaliana |
3.1.3.8 | monomer | 1 * 60000, SDS-PAGE | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.2 | AtPAP15 | - |
Arabidopsis thaliana |
3.1.3.2 | purple acid phosphatase | - |
Arabidopsis thaliana |
3.1.3.8 | AtPAP15 | - |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 23 | 37 | - |
Arabidopsis thaliana |
3.1.3.2 | 23 | 37 | highest activity | Arabidopsis thaliana |
3.1.3.8 | 23 | 37 | - |
Arabidopsis thaliana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 23 | 37 | most stable | Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 8.85 | - |
sodium phytate | - |
Arabidopsis thaliana | |
3.1.3.2 | 8.85 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 161.9 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
3.1.3.2 | 161.9 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 198 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 198.6 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
3.1.3.8 | 8.85 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 161.9 | - |
4-nitrophenylphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 198 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.2 | 4.5 | - |
- |
Arabidopsis thaliana |
3.1.3.8 | 4.5 | - |
- |
Arabidopsis thaliana |
EC Number | Organism | Comment | Expression |
---|---|---|---|
3.1.3.2 | Arabidopsis thaliana | expressed in the vasculature, pollen grains and roots | additional information |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.1.3.2 | physiological function | gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate | Arabidopsis thaliana |
3.1.3.8 | physiological function | gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate | Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.2 | 26.7 | - |
sodium phytate | - |
Arabidopsis thaliana | |
3.1.3.2 | 31.8 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 232 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 241.3 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
3.1.3.2 | 248 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.2 | 253.8 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
3.1.3.8 | 31.8 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 232 | - |
4-nitrophenylphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
3.1.3.8 | 248 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana |