Any feedback?
Literature summary extracted from
- Studies of a novel cysteine sulfoxide lyase from Petiveria alliacea: the first heteromeric alliinase (2009), Plant Physiol., 151, 1304-1316.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.4 | hydroxylamine | complete loss of enzyme activity | Petiveria alliacea |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.4 | additional information | - |
additional information | Km and Vmax vary as a function of substrate structure, with the most preferred substrates being the naturally occurring Petiveria alliacea compounds S-benzyl-L-cysteine sulfoxide and S-2-hydroxyethyl-L-cysteine sulfoxide | Petiveria alliacea | |
| 4.4.1.4 | 0.23 | - |
(2R)-2-amino-3-(phenethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 0.25 | - |
propiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 0.33 | - |
(2R)-2-amino-3-(ethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 0.39 | - |
petiveriin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 0.5 | - |
2-hydroxyethiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 0.72 | - |
(2R)-2-amino-3-(4-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 1.96 | - |
(2R)-2-amino-3-(phenylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 2.11 | - |
(2R)-2-amino-3-(4-chlorobenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 4.22 | - |
alliin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 9.29 | - |
(2R)-2-amino-3-(2-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 13.9 | - |
methiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 28.6 | - |
(2R)-2-amino-3-(1-phenylethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.4.1.4 | vacuole | compartmentalized in plant cell vacuoles, while its substrates, S-alk(en)yl-L-Cys sulfoxides, are located in the cytoplasm | Petiveria alliacea | 5773 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 145100 | - |
gel-filtration chromatography | Petiveria alliacea |
| 4.4.1.4 | 218700 | - |
SDS-PAGE, this is approximately 75000 Da higher than the molecular mass estimates of the alliinase molecule as measured by gel-filtration chromatography | Petiveria alliacea |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.4 | 2-hydroxyethiin | Petiveria alliacea | - |
(2-hydroxyethyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | petiveriin | Petiveria alliacea | - |
benzylsulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form petivericin. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.4 | Petiveria alliacea | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.4 | precipitation with ammonium sulfate, ion-exchange, hydroxyapatite, and gel-filtration chromatographies in sequence | Petiveria alliacea |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.4.1.4 | root | - |
Petiveria alliacea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 6.42 | - |
- |
Petiveria alliacea |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.4 | (2R)-2-amino-3-(1-phenylethylsulfinyl)propanoic acid | - |
Petiveria alliacea | (1-phenylethyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(2-methylbenzylsulfinyl)propanoic acid | - |
Petiveria alliacea | (2-methylbenzyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(4-chlorobenzylsulfinyl)propanoic acid | - |
Petiveria alliacea | (4-chlorobenzyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(4-methylbenzylsulfinyl)propanoic acid | - |
Petiveria alliacea | (4-methylbenzyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(ethylsulfinyl)propanoic acid | - |
Petiveria alliacea | (2R)-2-amino-3-(ethylsulfinyl)propanoic acid + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(phenethylsulfinyl)propanoic acid | - |
Petiveria alliacea | phenethylsulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | (2R)-2-amino-3-(phenylsulfinyl)propanoic acid | - |
Petiveria alliacea | phenylsulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | 2-hydroxyethiin | - |
Petiveria alliacea | (2-hydroxyethyl)sulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | alliin | i.e. (2R)-3-(allylsulfinyl)-2-aminopropanoic acid | Petiveria alliacea | 2-propene-thioic acid + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | isoalliin | - |
Petiveria alliacea | ? + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | methiin + H2O | - |
Petiveria alliacea | methanesulfenic acid + pyruvate + NH3 | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | petiveriin | - |
Petiveria alliacea | benzylsulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form petivericin. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
| 4.4.1.4 | propiin | - |
Petiveria alliacea | propylsulfanol + pyruvate + NH4+ | The sulfenic acid condenses with loss of water to form thiosulfinates. The alpha-aminoacrylic acid formed initially subsequently breaks down into pyruvate and ammonia | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.4 | pentamer | SDS-PAGE, two alpha-subunits (68.1 kDa each), one beta-subunit (56.0 kDa), one gamma-subunit (24.8 kDa), and one delta-subunit (13.9 kDa). The two alpha-subunits are connected by a disulfide bridge, and both alpha- and beta-subunits are glycosylated | Petiveria alliacea |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.4.1.4 | alliinase | - |
Petiveria alliacea |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 52 | - |
with the most preferred substrate petiveriin, Petiveria alliacea alliinase shows 80% higher activity at 37°C and 100% higher activity at 52°C than at ambient temperature (25°C). Even at 67°C, alliinase activity is still 19% higher than that observed at room temperature, indicating that the alliinase retains significant functionality over a broad range of temperature and possesses a high overall intrinsic thermal stability | Petiveria alliacea |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | - |
84 | - |
Petiveria alliacea |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 4 | 52 | alliinase activity increases with increasing temperature over a range of 4.0°C to 52°C. At 67°C, enzyme activity falls precipitously, and at 84°C, alliinase activity is completely lost, 10 min, pH 8.0 | Petiveria alliacea |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.4 | 1.75 | - |
(2R)-2-amino-3-(phenethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 1.96 | - |
propiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 2.48 | - |
(2R)-2-amino-3-(ethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 2.67 | - |
(2R)-2-amino-3-(4-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 4.2 | - |
2-hydroxyethiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 4.47 | - |
petiveriin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 6.48 | - |
(2R)-2-amino-3-(phenylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 8.18 | - |
(2R)-2-amino-3-(1-phenylethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 10.65 | - |
(2R)-2-amino-3-(4-chlorobenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 14.33 | - |
(2R)-2-amino-3-(2-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 15.1 | - |
methiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 20.67 | - |
alliin | pH 8.0, 22°C | Petiveria alliacea | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 8 | - |
- |
Petiveria alliacea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 4 | 11 | - |
Petiveria alliacea |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.4 | 5 | 10 | detectable activity | Petiveria alliacea |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.4.1.4 | Petiveria alliacea | chromatofocusing | - |
4.78 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.4 | 1.09 | - |
methiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 2.49 | - |
(2R)-2-amino-3-(1-phenylethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 13.4 | - |
(2R)-2-amino-3-(2-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 28.9 | - |
(2R)-2-amino-3-(phenylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 32.3 | - |
(2R)-2-amino-3-(4-methylbenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 42.8 | - |
alliin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 44 | - |
(2R)-2-amino-3-(4-chlorobenzylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 65.7 | - |
(2R)-2-amino-3-(ethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 66.5 | - |
(2R)-2-amino-3-(phenethylsulfinyl)propanoic acid | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 68.8 | - |
propiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 73.4 | - |
2-hydroxyethiin | pH 8.0, 22°C | Petiveria alliacea | |
| 4.4.1.4 | 100 | - |
petiveriin | pH 8.0, 22°C | Petiveria alliacea | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
