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Literature summary extracted from

  • Sullivan, M.
    A novel red clover hydroxycinnamoyl transferase has enzymatic activities consistent with a role in phaselic acid biosynthesis (2009), Plant Physiol., 150, 1866-1879.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.133 expressed in Escherichia coli Trifolium pratense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.133 48300
-
 calculated from cDNA Trifolium pratense

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.133 Trifolium pratense B6D7P1 cDNA HCT1A (almost identical to HCT1B)
-
2.3.1.133 Trifolium pratense B6D7P2
-
-
2.3.1.133 Trifolium pratense B6DQK4 cDNA HCT1B (almost identical to HCT1A)
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.133 leaf expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves Trifolium pratense
-
2.3.1.133 leaf HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems Trifolium pratense
-
2.3.1.133 stem expression analyses indicate that HCT1 mRNA accumulates to 4fold higher levels in stems than in leaves Trifolium pratense
-
2.3.1.133 stem HCT2 mRNA accumulates to 10fold higher levels in leaves than in stems Trifolium pratense
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.133 4-coumaroyl-CoA + L-malate
-
 Trifolium pratense CoA + 2-O-(4-coumaryl)-L-malate
-
 ?
2.3.1.133 4-coumaroyl-CoA + quinate reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor Trifolium pratense CoA + 4-coumaroylquinate
-
 ?
2.3.1.133 4-coumaroyl-CoA + shikimate
-
 Trifolium pratense CoA + 5-O-(4-coumaroyl)shikimate
-
 ?
2.3.1.133 caffeoyl-CoA + L-malate
-
 Trifolium pratense CoA + 2-O-caffeoyl-L-malate
-
 ?
2.3.1.133 caffeoyl-CoA + quinate reaction products are detected when quinic acid is used as an acceptor, although the amount of product formed is less than 2% of that formed when shikimic acid serves as the acceptor Trifolium pratense CoA + caffeoylquinate
-
 ?
2.3.1.133 caffeoyl-CoA + shikimate
-
 Trifolium pratense CoA + 5-O-caffeoylshikimate
-
 ?
2.3.1.133 additional information no reaction products are detected when HCT1 and either hydroxycinnamoyl-CoA derivative are incubated with malic acid, Glc, or L-DOPA as acceptor Trifolium pratense ?
-
 ?
2.3.1.133 additional information no reaction products are detected when shikimic acid, quinic acid, Glc, or L-DOPA is used as an acceptor Trifolium pratense ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.133 HCT1 two cDNAs are isolated HCT1A and HCT1B which are nearly identical Trifolium pratense
2.3.1.133 HCT2
-
 Trifolium pratense
2.3.1.133 hydroxycinnamoyltransferase
-
 Trifolium pratense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.133 30
-
 assay at Trifolium pratense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.133 7.5
-
 assay at Trifolium pratense

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.133 6.1 6.7 optimal pH range with malate as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 6.1 and 6.7 using sodium phosphate buffer Trifolium pratense
2.3.1.133 7.5 7.9 optimal pH range with shikimic acid as the acceptor, highest reaction rates are observed for both hydroxycinnamoyl-CoA derivatives between pH 7.5 and 7.9 using sodium phosphate buffer Trifolium pratense