BRENDA - Enzyme Database show

A novel lipoxygenase in pea roots. Its function in wounding and biotic stress

Veronico, P.; Giannino, D.; Melillo, M.T.; Leone, A.; Reyes, A.; Kennedy, M.W.; Bleve-Zacheo, T.; Plant Physiol. 141, 1045-1055 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.B6
expression in Pichia pastoris
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.B6
94000
-
x * 94000, SDS-PAGE
Pisum sativum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.B6
Pisum sativum
Q14ST9
subsp. transcaucasicum Govorov
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.B6
root
LOXN2 message is widespread in the cortex and endodermis of healthy roots, but specifically localized at high level in the cells bordering the nematode-induced syncytia of infected roots. In uninfected roots, the transcript is visualized in cortical and endodermal cells. In resistant infected roots, faint and infrequent signal spots are observed in the cortex, but LOXN2 message is abundant in the outer cells surrounding the vascular cylinder, in those cells injured by nematode penetration and those flanking the induced syncytia. In susceptible infected roots, the transcript is detected in cortical cells damaged by nematode invasion and undergoing necrosis, and in parenchymatous cells of the vascular cylinder strictly related to syncytia development
Pisum sativum
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.B6
1.35
-
activity with linoleate
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B6
arachidonate + O2
specific activity is 13% of the activity with linoleate
706289
Pisum sativum
?
products not determined
-
-
?
1.13.11.B6
linoleate + O2
-
706289
Pisum sativum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the ratio of 9-hydroperoxy-(10E,12Z)-octadecadienoate to 13-hydroperoxy-(10E,12Z)-octadecadienoate is 3:1
-
-
?
1.13.11.B6
linolenate + O2
specific activity is 17% of the activity with linoleate. The R/S stereoconfiguration of the product is not determined
706289
Pisum sativum
?
products not determined
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.B6
?
x * 94000, SDS-PAGE
Pisum sativum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.B6
expression in Pichia pastoris
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.B6
94000
-
x * 94000, SDS-PAGE
Pisum sativum
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.B6
root
LOXN2 message is widespread in the cortex and endodermis of healthy roots, but specifically localized at high level in the cells bordering the nematode-induced syncytia of infected roots. In uninfected roots, the transcript is visualized in cortical and endodermal cells. In resistant infected roots, faint and infrequent signal spots are observed in the cortex, but LOXN2 message is abundant in the outer cells surrounding the vascular cylinder, in those cells injured by nematode penetration and those flanking the induced syncytia. In susceptible infected roots, the transcript is detected in cortical cells damaged by nematode invasion and undergoing necrosis, and in parenchymatous cells of the vascular cylinder strictly related to syncytia development
Pisum sativum
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.13.11.B6
1.35
-
activity with linoleate
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.B6
arachidonate + O2
specific activity is 13% of the activity with linoleate
706289
Pisum sativum
?
products not determined
-
-
?
1.13.11.B6
linoleate + O2
-
706289
Pisum sativum
(10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate
the ratio of 9-hydroperoxy-(10E,12Z)-octadecadienoate to 13-hydroperoxy-(10E,12Z)-octadecadienoate is 3:1
-
-
?
1.13.11.B6
linolenate + O2
specific activity is 17% of the activity with linoleate. The R/S stereoconfiguration of the product is not determined
706289
Pisum sativum
?
products not determined
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.B6
?
x * 94000, SDS-PAGE
Pisum sativum
Expression
EC Number
Organism
Commentary
Expression
1.13.11.B6
Pisum sativum
LOXN2 transcription is monitored in roots after mechanical injury and during nematode infection. The message peaks at 3 and 24 h after wounding in both genotypes and is more abundant in the resistant than in the susceptible pea. In nematode-infected roots, transcription of several LOX genes is triggered except LOXN2, which is repressed in both genotypes
down
General Information
EC Number
General Information
Commentary
Organism
1.13.11.B6
physiological function
LOXN2 may be involved in late mechanisms of host resistance
Pisum sativum
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.B6
physiological function
LOXN2 may be involved in late mechanisms of host resistance
Pisum sativum
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.13.11.B6
Pisum sativum
LOXN2 transcription is monitored in roots after mechanical injury and during nematode infection. The message peaks at 3 and 24 h after wounding in both genotypes and is more abundant in the resistant than in the susceptible pea. In nematode-infected roots, transcription of several LOX genes is triggered except LOXN2, which is repressed in both genotypes
down