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Literature summary extracted from

  • Stratilova, E.; Ait-Mohand, F.; Rehulka, P.; Garajova, S.; Flodrova, D.; Rehulkova, H.; Farkas, V.
    Xyloglucan endotransglycosylases (XETs) from germinating nasturtium (Tropaeolum majus) seeds: Isolation and characterization of the major form (2010), Plant Physiol. Biochem., 48, 207-215.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.207 xyloglucan-derived oligosaccharide
-
 Tropaeolum majus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.207 26500
-
 deglycosylated major enzyme form XET(6.3), SDS-PAGE Tropaeolum majus
2.4.1.207 29000
-
 glycosylated major enzyme form XET(6.3), SDS-PAGE Tropaeolum majus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.207 Tropaeolum majus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.207 glycoprotein
-
 Tropaeolum majus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.207 polyanion SI column chromatography, Spheron phosphate column chromatography, and Superdex 75 gel filtration Tropaeolum majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.207 seed
-
 Tropaeolum majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.207 donor xyloglucan + acceptor beta-(1,4)-xylooligosaccharide the transglycosylating activity is 0.31% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor beta-(1,6)-D-gluco-oligosaccharide the transglycosylating activity is 0.69% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor cellooligosaccharide the transglycosylating activity is 4.6% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor laminarioligosaccharide the transglycosylating activity is 0.23% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor mixed-linkage beta-(1,3 1,4)-gluco-oligosaccharide the transglycosylating activity is 2.06% of that determined with xyloglucan oligosaccharide as acceptor Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor pustulooligosaccharide
-
 Tropaeolum majus ?
-
 ?
2.4.1.207 donor xyloglucan + acceptor xyloglucan
-
 Tropaeolum majus ?
-
 ?
2.4.1.207 additional information the enzyme exhibits broad substrate specificity by transferring xyloglucan or hydroxyethylcellulose fragments not only to oligoxyloglucosides and cellooligosaccharides but also to oligosaccharides derived from beta-(1,4)-D-glucuronoxylan, beta-(1,6)-D-glucan, mixed-linkage beta-(1,3 1,4)-D-glucan and at a relatively low rate also to beta-(1,3)-gluco-oligosaccharides Tropaeolum majus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.207 XET(6.3) major enzyme form, XET(6.3) is classified into group II of the XTH phylogeny of glycoside hydrolase family GH16 Tropaeolum majus
2.4.1.207 XTH
-
 Tropaeolum majus
2.4.1.207 xyloglucan endotransglycosylase
-
 Tropaeolum majus
2.4.1.207 xyloglucan endotransglycosylase/hydrolase
-
 Tropaeolum majus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.207 42
-
-
 Tropaeolum majus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.207 38 50 the activity of XETs from nasturtium seeds is stable at 40°C during 2 h (at 38°C during 8 h), but it is completely lost at 50°C Tropaeolum majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.207 5 8.5
-
 Tropaeolum majus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.207 Tropaeolum majus major enzyme form XET(6.3), isoelectric focusing
-
 6.3