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Literature summary extracted from

  • Day, A.; Neutelings, G.; Nolin, F.; Grec, S.; Habrant, A.; Cronier, D.; Maher, B.; Rolando, C.; David, H.; Chabbert, B.; Hawkins, S.
    Caffeoyl coenzyme A O-methyltransferase down-regulation is associated with modifications in lignin and cell-wall architecture in flax secondary xylem (2009), Plant Physiol. Biochem., 47, 9-19.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Linum usitatissimum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.104 additional information enzyme downregulation leads to reduced lignin quantity a modified syringyl/guaiacyl lignin monomer ratio in the non-condensed lignin fraction, together with altered xylem organization, reduced cell-wall thickness and the appearance of an irregular xylem phenotype, phentypes of two independently down-regulated lines, overview Linum usitatissimum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.0015
-
 caffeoyl-CoA recombinant enzyme, 30°C Linum usitatissimum
2.1.1.104 0.039
-
 caffeic acid recombinant enzyme, 30°C Linum usitatissimum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.104 27900
-
 x * 27900, about, sequence calculation Linum usitatissimum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.104 additional information Linum usitatissimum CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA Linum usitatissimum
-
 S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid Linum usitatissimum
-
 S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Linum usitatissimum
-
 S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Linum usitatissimum Q4JHB1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3), removal of the His-tag Linum usitatissimum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 stem
-
 Linum usitatissimum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information CCoAOMT down-regulation induces changes in xylem cell-wall structure and the lignin fractions Linum usitatissimum ?
-
 ?
2.1.1.104 additional information flax CCoAOMT possesses a small, but probably significant 5' methylating activity, in addition to a more usual 3' methylating activity Linum usitatissimum ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA
-
 Linum usitatissimum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxy-feruloyl-CoA 3' methylating activity Linum usitatissimum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeic acid
-
 Linum usitatissimum S-adenosyl-L-homocysteine + ferulic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Linum usitatissimum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA preferred substrate, 3' methylating activity Linum usitatissimum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.104 ? x * 27900, about, sequence calculation Linum usitatissimum

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 caffeoyl coenzyme A O-methyltransferase
-
 Linum usitatissimum
2.1.1.104 CCoAOMT
-
 Linum usitatissimum
2.1.1.104 More the enzyme belongs to the O-methyltransferase superfamily Linum usitatissimum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.104 30
-
 assay at Linum usitatissimum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.104 S-adenosyl-L-methionine
-
 Linum usitatissimum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.104 Linum usitatissimum sequence calculation
-
 5.3

General Information

EC Number General Information Comment Organism
2.1.1.104 metabolism the enzyme catalyzes central essential reactions in the lignin biosynthesis, pathway overview Linum usitatissimum