Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yang, X.; Sun, W.; Liu, J.P.; Liu, Y.J.; Zeng, Q.Y.
    Biochemical and physiological characterization of a tau class glutathione transferase from rice (Oryza sativa) (2009), Plant Physiol. Biochem., 47, 1061-1068.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.18 tau class GST gene OsGSTU17, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged GSTU17 in Escherichia coli strain Bl21 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.058
-
 glutathione pH 7.4, 37°C Oryza sativa
2.5.1.18 0.324
-
 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole pH 7.4, 37°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 25180
-
 x * 25180, GSTU17, sequence calculation Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Oryza sativa Q93WY5 GSTU17; isozyme GSTU17
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 bud low expression of GSTU17 Oryza sativa
-
2.5.1.18 leaf high expression of GSTU17 in mature, lower in young leaves, no expression in immature leaves Oryza sativa
-
2.5.1.18 root low expression of GSTU17 Oryza sativa
-
2.5.1.18 stem low expression of GSTU17 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 0.203
-
 GSTU17, substrate 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 glutathione + 1-chloro-2,4-dinitrobenzene
-
 Oryza sativa S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 glutathione + 4-nitrobenzo-2-oxa-1,3-diazole
-
 Oryza sativa ?
-
 ?
2.5.1.18 glutathione + 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole
-
 Oryza sativa ?
-
 ?
2.5.1.18 glutathione + cumene hydroperoxide
-
 Oryza sativa ?
-
 ?
2.5.1.18 additional information no activity with 1,2-dichloro-4-nitrobenzene, 4-nitrophenyl acetate, and ethacrynic acid Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 ? x * 25180, GSTU17, sequence calculation Oryza sativa
2.5.1.18 More structure homology modeling Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
2.5.1.18 GSTU17
-
 Oryza sativa
2.5.1.18 tau class glutathione transferase
-
 Oryza sativa
2.5.1.18 tau class GST
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.18 35 40
-
 Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.18 25 65
-
 Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.18 0.257
-
 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole pH 7.4, 37°C Oryza sativa
2.5.1.18 0.264
-
 glutathione pH 7.4, 37°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.5
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 5.5 8.5 activity range of GSTU17 Oryza sativa

General Information

EC Number General Information Comment Organism
2.5.1.18 metabolism the classical phase II detoxification glutathione transferases are key metabolic enzymes that catalyze the conjugation of glutathione to various electrophilic compounds Oryza sativa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.18 0.013
-
 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole pH 7.4, 37°C Oryza sativa
2.5.1.18 0.076
-
 glutathione pH 7.4, 37°C Oryza sativa