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Literature summary extracted from

  • Wang, R.; Shen, W.; Liu, L.; Jiang, L.; Liu, Y.; Su, N.; Wan, J.
    A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack (2008), Plant Mol. Biol., 66, 401-414.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.11.12 additional information the manipulating of the expression of the LOX gene may provide a basis for advancing both the fundamental and applied biology of rice Oryza sativa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.B6 expression in Escherichia coli Oryza sativa
1.13.11.12 LOX1 ORF cloned into vector pET30a(+) for overexpression in Escherichia coli. LOX1-green GFP fusion construct, under the control of the cauliflower mosaic virus 35S promoter, expressed in Arabidopsis plants. Transgenic rice lines carrying either sense or antisense constructs under the control of a cauliflower mosaic virus 35S promoter Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.13.11.12 chloroplast
-
Oryza sativa 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.B6 105000
-
1 * 105000, recombinant enzyme including His-tag, SDS-PAGE Oryza sativa
1.13.11.12 105000
-
1 * 105000, SDS-PAGE, His-tagged enzyme Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.B6 linoleate + O2 Oryza sativa
-
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:3 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.B6 Oryza sativa Q27PX2 subsp. japonica, variety Beilu PL2
-
1.13.11.12 Oryza sativa Q27PX2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.12 soluble LOX purified with His bind kits Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.B6 seed OsLOX1 transcripts are detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding Oryza sativa
-
1.13.11.B6 seedling OsLOX1 transcripts are detected at low abundance in immature seeds and newly germinated seedlings, but accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding Oryza sativa
-
1.13.11.12 seed low abundance in immature seeds Oryza sativa
-
1.13.11.12 seedling low abundance in newly germinated seedlings Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.B6 linoleate + O2
-
Oryza sativa (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate is 4:3 ?
1.13.11.B6 linoleate + O2
-
Oryza sativa (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate + (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate dual positional specificity. The ratio of (10E,12Z)-9-hydroperoxy-10,12-octadecadienoate to (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate is 4:3. Both products are predominantly in the S configuration ?
1.13.11.12 linoleate + O2
-
Oryza sativa (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate
-
?

Subunits

EC Number Subunits Comment Organism
1.13.11.B6 monomer 1 * 105000, recombinant enzyme including His-tag, SDS-PAGE Oryza sativa
1.13.11.12 monomer 1 * 105000, SDS-PAGE, His-tagged enzyme Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.13.11.B6 OsLOX1
-
Oryza sativa
1.13.11.12 linoleate:oxygen oxidoreductase
-
Oryza sativa
1.13.11.12 LOX1
-
Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.B6 4.8
-
-
Oryza sativa
1.13.11.12 4.8
-
-
Oryza sativa

Expression

EC Number Organism Comment Expression
1.13.11.B6 Oryza sativa transformants with a lower level of OsLOX1 expression are less able to tolerate brown planthopper attack down
1.13.11.B6 Oryza sativa transformants with enhanced level of OsLOX1 expression are more resistant to brown planthopper attack up
1.13.11.12 Oryza sativa in all antisense transgenic rice lines and more than half of the sense lines the expression levels of LOX1, the levels of enzyme activity, and the levels of the endogenous LOX1 products (jasmonic acid, (Z)-3-hexenal and colneleic acid) at 6, 48, and 48 h after brown planthopper feeding respectively, are below the levels found in non-transgenic control plants down
1.13.11.12 Oryza sativa LOX1 transcripts accumulate rapidly and transiently in response to wounding or brown planthopper attack, reaching a peak 3 h after wounding and 6 h after insect feeding. Transgenic rice lines show altered LOX1 activity up

General Information

EC Number General Information Comment Organism
1.13.11.B6 physiological function OsLOX1 is likely to be the main LOX involved in rice response to biotic and wounding stress. 13-LOX pathway products (such as (Z)-3-hexenyl acetate) protect plants against herbivores by inducing the production of several defence molecules and by attracting herbivore predators. products of 9-LOXs might participate in plant resistance to insect attack, as well Oryza sativa
1.13.11.12 physiological function LOX1 is a non-conventional LOX with 13- or dual position-specific LOX activity. LOX1 is involved in the synthesis of jasmonic acid, colneleic acid, and (Z)-3-hexenal. The LOX1 product is involved in tolerance of the rice plant to wounding and brown planthopper Nilaparvata lugens attack Oryza sativa