Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Galant, A.; Arkus, K.A.; Zubieta, C.; Cahoon, R.E.; Jez, J.M.
    Structural basis for evolution of product diversity in soybean glutathione biosynthesis (2009), Plant Cell, 21, 3450-3458.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.23 expression in Escherichia coli BL21 Glycine max

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.23 vapor diffusion method Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.23 L487A improved catalytic efficiency using glcyine Glycine max
6.3.2.23 L487A/P488A conversion of substrate preference from beta-alanine to glycine Glycine max
6.3.2.23 P488A improved catalytic efficiency using glcyine Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.23 additional information
-
 additional information glycine, value above 100 mM, wild-type enzyme Glycine max
6.3.2.23 0.023
-
 ATP
-
 Glycine max
6.3.2.23 0.044
-
 gamma-L-glutamyl-L-cysteine
-
 Glycine max
6.3.2.23 2.2
-
 glycine mutant L487A/P488A Glycine max
6.3.2.23 3.39
-
 beta-Alanine wild-type enzyme Glycine max
6.3.2.23 3.8
-
 beta-Alanine mutant L487A Glycine max
6.3.2.23 5.1
-
 glycine mutant P488A Glycine max
6.3.2.23 6.5
-
 glycine mutant L487A Glycine max
6.3.2.23 8
-
 beta-Alanine mutant P488A Glycine max
6.3.2.23 24.8
-
 beta-Alanine mutant L487A/P488A Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.23 50000
-
 SDS-PAGE Glycine max

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.23 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.23 mixed benzamidine-sepharose/Ni2+-NTA column, Superdex-200 FPLC column Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + beta-alanine assay at pH 7.5, 25°C Glycine max ?
-
 ?
6.3.2.23 ATP + gamma-L-glutamyl-L-cysteine + glycine assay at pH 7.5, 25°C Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.23 monomer
-
 Glycine max

Synonyms

EC Number Synonyms Comment Organism
6.3.2.23 hGS
-
 Glycine max
6.3.2.23 homoglutathione synthetase
-
 Glycine max

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.3.2.23 0.001
-
 glycine wild-type enzyme Glycine max
6.3.2.23 0.046
-
 glycine mutant L487A Glycine max
6.3.2.23 0.077
-
 beta-Alanine mutant L487A/P488A Glycine max
6.3.2.23 0.211
-
 beta-Alanine mutant L487A Glycine max
6.3.2.23 0.274
-
 glycine mutant P488A Glycine max
6.3.2.23 0.313
-
 beta-Alanine mutant P488A Glycine max
6.3.2.23 0.708
-
 beta-Alanine wild-type enzyme Glycine max
6.3.2.23 0.95
-
 glycine mutant L487A/P488A Glycine max
6.3.2.23 56.82
-
 gamma-L-glutamyl-L-cysteine
-
 Glycine max
6.3.2.23 73.91
-
 ATP
-
 Glycine max