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Literature summary extracted from

  • Schneider, T.; Keller, F.
    Raffinose in chloroplasts is synthesized in the cytosol and transported across the chloroplast envelope (2009), Plant Cell Physiol., 50, 2174-2182.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.82 cytosol
-
 Spinacia oleracea 5829
-
2.4.1.82 cytosol
-
 Arabidopsis thaliana 5829
-
2.4.1.82 cytosol
-
 Ajuga reptans 5829
-
2.4.1.82 protoplast
-
 Spinacia oleracea
-
-
2.4.1.82 protoplast
-
 Arabidopsis thaliana
-
-
2.4.1.82 protoplast
-
 Ajuga reptans
-
-
2.4.1.123 cytosol
-
 Spinacia oleracea 5829
-
2.4.1.123 cytosol
-
 Arabidopsis thaliana 5829
-
2.4.1.123 cytosol
-
 Ajuga reptans 5829
-
2.4.1.123 protoplast
-
 Spinacia oleracea
-
-
2.4.1.123 protoplast
-
 Arabidopsis thaliana
-
-
2.4.1.123 protoplast
-
 Ajuga reptans
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol Spinacia oleracea
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?
2.4.1.123 UDP-galactose + myo-inositol Arabidopsis thaliana
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?
2.4.1.123 UDP-galactose + myo-inositol Ajuga reptans
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.82 Ajuga reptans
-
-
-
2.4.1.82 Ajuga reptans
-
 plants are cold-treated
-
2.4.1.82 Arabidopsis thaliana
-
-
-
2.4.1.82 Arabidopsis thaliana
-
 plants are cold-treated
-
2.4.1.82 Spinacia oleracea
-
-
-
2.4.1.82 Spinacia oleracea
-
 plants are cold-treated
-
2.4.1.123 Ajuga reptans
-
-
-
2.4.1.123 Arabidopsis thaliana
-
-
-
2.4.1.123 Spinacia oleracea
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.82 leaf
-
 Spinacia oleracea
-
2.4.1.82 leaf
-
 Arabidopsis thaliana
-
2.4.1.82 leaf
-
 Ajuga reptans
-
2.4.1.82 mesophyll
-
 Spinacia oleracea
-
2.4.1.82 mesophyll
-
 Arabidopsis thaliana
-
2.4.1.82 mesophyll
-
 Ajuga reptans
-
2.4.1.123 leaf
-
 Spinacia oleracea
-
2.4.1.123 leaf
-
 Arabidopsis thaliana
-
2.4.1.123 leaf
-
 Ajuga reptans
-
2.4.1.123 mesophyll
-
 Spinacia oleracea
-
2.4.1.123 mesophyll
-
 Arabidopsis thaliana
-
2.4.1.123 mesophyll
-
 Ajuga reptans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.82 additional information
-
 no enzyme activity is found in chloroplasts Spinacia oleracea
2.4.1.82 additional information
-
 no enzyme activity is found in chloroplasts Arabidopsis thaliana
2.4.1.82 additional information
-
 no enzyme activity is found in chloroplasts Ajuga reptans
2.4.1.82 0.00138
-
 protoplast Ajuga reptans
2.4.1.82 0.00258
-
 protoplast Arabidopsis thaliana
2.4.1.82 0.00396
-
 protoplast Spinacia oleracea
2.4.1.123 additional information
-
 no enzyme activity is found in chloroplasts Spinacia oleracea
2.4.1.123 additional information
-
 no enzyme activity is found in chloroplasts Arabidopsis thaliana
2.4.1.123 additional information
-
 no enzyme activity is found in chloroplasts Ajuga reptans
2.4.1.123 0.00114
-
 protoplast Spinacia oleracea
2.4.1.123 0.02826
-
 protoplast Ajuga reptans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.82 myo-inositol + raffinose
-
 Spinacia oleracea 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
 r
2.4.1.82 myo-inositol + raffinose
-
 Arabidopsis thaliana 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
 r
2.4.1.82 myo-inositol + raffinose
-
 Ajuga reptans 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
 r
2.4.1.123 UDP-galactose + myo-inositol
-
 Spinacia oleracea UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?
2.4.1.123 UDP-galactose + myo-inositol
-
 Arabidopsis thaliana UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?
2.4.1.123 UDP-galactose + myo-inositol
-
 Ajuga reptans UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.82 raffinose synthase
-
 Spinacia oleracea
2.4.1.82 raffinose synthase
-
 Arabidopsis thaliana
2.4.1.82 raffinose synthase
-
 Ajuga reptans
2.4.1.82 RafS
-
 Spinacia oleracea
2.4.1.82 RafS
-
 Arabidopsis thaliana
2.4.1.82 RafS
-
 Ajuga reptans
2.4.1.123 galactinol synthase
-
 Spinacia oleracea
2.4.1.123 galactinol synthase
-
 Arabidopsis thaliana
2.4.1.123 galactinol synthase
-
 Ajuga reptans
2.4.1.123 GOLS
-
 Spinacia oleracea
2.4.1.123 GOLS
-
 Arabidopsis thaliana
2.4.1.123 GOLS
-
 Ajuga reptans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.82 6.5
-
 assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Spinacia oleracea
2.4.1.82 6.5
-
 assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Arabidopsis thaliana
2.4.1.82 6.5
-
 assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Ajuga reptans
2.4.1.123 7.5
-
 assay at, GolS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Spinacia oleracea
2.4.1.123 7.5
-
 assay at, GolS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Arabidopsis thaliana
2.4.1.123 7.5
-
 assay at, GolS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Ajuga reptans

General Information

EC Number General Information Comment Organism
2.4.1.82 physiological function enzyme is involved in stress resistance Spinacia oleracea
2.4.1.82 physiological function enzyme is involved in stress resistance Arabidopsis thaliana
2.4.1.82 physiological function enzyme is involved in stress resistance Ajuga reptans
2.4.1.123 physiological function enzyme is involved in stress resistance Spinacia oleracea
2.4.1.123 physiological function enzyme is involved in stress resistance Arabidopsis thaliana
2.4.1.123 physiological function enzyme is involved in stress resistance Ajuga reptans