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Literature summary extracted from

  • Flores-Sanchez, I.J.; Verpoorte, R.
    PKS activities and biosynthesis of cannabinoids and flavonoids in Cannabis sativa L. plants (2008), Plant Cell Physiol., 49, 1767-1782.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.206 additional information the goal for engineering of zero-cannabinoid plants is knocking out the first step of the biosynthesis catalyzed by the type III PKS enzyme Cannabis sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA Cannabis sativa
-
 3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2 formation of olivetolic acid ?
2.3.1.206 additional information Cannabis sativa no formation of olivetol by an enzymatic or chemical decarboxylation from olivetolic acid, cannabinoid profiling, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.156 Cannabis sativa
-
-
-
2.3.1.206 Cannabis sativa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.156 flower high activity is found in male flowers Cannabis sativa
-
2.3.1.156 fruit
-
 Cannabis sativa
-
2.3.1.156 seedling
-
 Cannabis sativa
-
2.3.1.156 whole plant
-
 Cannabis sativa
-
2.3.1.206 bract
-
 Cannabis sativa
-
2.3.1.206 flower female Cannabis sativa
-
2.3.1.206 leaf female and male Cannabis sativa
-
2.3.1.206 additional information no enzyme activity in seedlings, fruits, and roots. Neither cannabinoid acids nor their decarboxylated forms are found in seedlings and roots in the four varieties of cannabis plants. Accumulation of cannabinoids in bracts during the growth and development of glandular trichomes from flowers Cannabis sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.156 additional information
-
 following the activity through the development of the glandular trichomes on the female flowers phlorisovalerophenone synthase shows the highest activity at 21-29 days Cannabis sativa
2.3.1.206 0.0024
-
 highest tissue activity, in bracts, pH 7.0, 30°C Cannabis sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.156 isovaleryl-CoA + malonyl-CoA
-
 Cannabis sativa phloroisovalerophenone + CO2 + CoA
-
 ?
2.3.1.206 3 malonyl-CoA + hexanoyl-CoA
-
 Cannabis sativa 3 CoA + 3,5,7-trioxododecanoyl-CoA + 3 CO2 formation of olivetolic acid ?
2.3.1.206 additional information no formation of olivetol by an enzymatic or chemical decarboxylation from olivetolic acid, cannabinoid profiling, overview Cannabis sativa ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.156 phlorisovalerophenone synthase
-
 Cannabis sativa
2.3.1.156 VPS
-
 Cannabis sativa
2.3.1.206 olivetol synthase
-
 Cannabis sativa
2.3.1.206 olivetol-forming PKS
-
 Cannabis sativa
2.3.1.206 PKS
-
 Cannabis sativa
2.3.1.206 type III PKS
-
 Cannabis sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.156 30
-
 assay at Cannabis sativa
2.3.1.206 30
-
 assay at Cannabis sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.156 7
-
 assay at Cannabis sativa
2.3.1.206 7
-
 assay at Cannabis sativa

General Information

EC Number General Information Comment Organism
2.3.1.206 metabolism the enzyme is involved the biosynthesis of the first precursor of cannabinoids, olivetolic acid. Cannabinoid and flavonoid profiling, overview. Accumulation of cannabinoids in bracts during the growth and development of glandular trichomes from flowers, cannabinoid accumulation iscorrelated with maximum activities for an olivetol-forming PKS, developmental and defens-related regulation of cannabinoid pathway enzymes, overview Cannabis sativa