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Literature summary extracted from

  • Lilley, R.M.; Ralph, P.J.; Larkum, A.W.
    The Determination of Activity of the Enzyme Rubisco in Cell Extracts of the Dinoflagellate Alga Symbiodinium sp. by Manganese Chemiluminescence and its Response to Short-term Thermal Stress of the Alga (2010), Plant Cell Environ., 33, 995-1004.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.39 molecular biology a continuous assay for Rubisco activity in crude cell extracts using the Mn2+ chemiluminescence of Rubisco oxygenase is described Symbiodinium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.39 Mn2+
-
 Symbiodinium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Symbiodinium sp. Q41406
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 crude cell extracts bind [14C]2-carboxy-D-arabitinol 1,5-bisphosphate (CABP) to a high molecular mass fraction and CABP-Rubisco is separated by gel filtration chromatography Symbiodinium sp.

Storage Stability

EC Number Storage Stability Organism
4.1.1.39 -80°C, the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly Symbiodinium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-Ribulose 1,5-bisphosphate + CO2
-
 Symbiodinium sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 Ribulose 1,5-bisphosphate carboxylase/oxygenase
-
 Symbiodinium sp.
4.1.1.39 Rubisco
-
 Symbiodinium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.39 20.7
-
 assay at Symbiodinium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.39 -80
-
 the activity of fast-frozen cell extracts is stable at -80°C and, after thawing and storage on ice, remain stable for up to 1 h before declining non-linearly Symbiodinium sp.
4.1.1.39 36
-
 after pre-treatment of Symbiodinium cell cultures in darkness at temperatures above 30°C, the extracted Rubisco activities decrease, with almost complete loss of activity above 36°C Symbiodinium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.39 8.1
-
 assay at Symbiodinium sp.