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Literature summary extracted from

  • Singh, K.; Kumar, S.; Yadav, S.; Ahuja, P.
    Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze] (2009), Plant Biotechnol. Rep., 3, 95-101.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 expression in Escherichia coli Camellia sinensis
1.1.1.219 into expression vector pQE-30 UA, and transformed into Escherichia coli M15 pREP-4 competent cells Camellia sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.219 abscisic acid decreases expression Camellia sinensis
1.1.1.219 additional information is unaffected by gibberellic acid treatment Camellia sinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.219 membrane
-
 Camellia sinensis 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.219 38670
-
 sequence analysis Camellia sinensis
1.1.1.219 38670
-
 x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide Camellia sinensis
1.1.1.219 41000
-
 x * 41000, SDS-PAGE Camellia sinensis
1.1.1.219 41000
-
 x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide Camellia sinensis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Camellia sinensis Q6DT40
-
-
1.1.1.219 Camellia sinensis Q9S787
-
-
1.1.1.219 Camellia sinensis (L.) O. Kuntze Q6DT40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.219 on Ni-NTA column Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 leaf expression is maximum in younger rather than older leaves Camellia sinensis
-
1.1.1.219 leaf expression is maximal in younger rather than older leaves Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.219 42.8
-
 pH 7.0, 25°C Camellia sinensis
1.1.1.219 42850
-
 recombinant protein Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (+)-dihydroquercetin + NADPH + H+
-
 Camellia sinensis cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 (+)-dihydroquercetin + NADPH + H+
-
 Camellia sinensis (L.) O. Kuntze cis-3,4-leucocyanidin + NADP+
-
 ?
1.1.1.219 dihydroquercetin + NADPH + H+
-
 Camellia sinensis leucocyanidin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.219 ? x * 41000, SDS-PAGE Camellia sinensis
1.1.1.219 ? x * 38670, calculated, x * 41000, SDS-PAGE of recombinant protein with fusion peptide Camellia sinensis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Camellia sinensis
1.1.1.219 dihydroflavonol 4-reductase
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Camellia sinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.219 Camellia sinensis calculated
-
 6.2
1.1.1.219 Camellia sinensis sequence analysis
-
 6.22

Expression

EC Number Organism Comment Expression
1.1.1.219 Camellia sinensis in response to drought stress down
1.1.1.219 Camellia sinensis expression is down-regulated in response to drought stress and abscisic acid, and unaffected by gibberellic acid treatment down
1.1.1.219 Camellia sinensis in response to wounding, with concomitant modulation of catechins content. Expression is maximum in younger rather than older leaves up
1.1.1.219 Camellia sinensis expression is up-regulated in response to wounding, with concomitant modulation of catechins content up