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Literature summary extracted from

  • Liedschulte, V.; Wachter, A.; Zhigang, A.; Rausch, T.
    Exploiting plants for glutathione (GSH) production: Uncoupling GSH synthesis from cellular controls results in unprecedented GSH accumulation (2010), Plant Biotechnol. J., 8, 1-14.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 expression in Escherichia coli, transformation into tobacco plants Streptococcus thermophilus
6.3.2.3 expression in Escherichia coli, transformation into tobacco plants Streptococcus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.2 0.075
-
ATP
-
Streptococcus thermophilus
6.3.2.2 0.399
-
L-cysteine
-
Streptococcus thermophilus
6.3.2.2 33
-
L-glutamate
-
Streptococcus thermophilus
6.3.2.3 0.083
-
ATP
-
Streptococcus thermophilus
6.3.2.3 0.762
-
gamma-L-glutamyl-L-cysteine
-
Streptococcus thermophilus
6.3.2.3 4.33
-
glycine
-
Streptococcus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.2 cytosol
-
Streptococcus thermophilus 5829
-
6.3.2.2 plastid
-
Streptococcus thermophilus 9536
-
6.3.2.3 cytosol
-
Streptococcus thermophilus 5829
-
6.3.2.3 plastid
-
Streptococcus thermophilus 9536
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.2 80000
-
SDS-PAGE Streptococcus thermophilus
6.3.2.3 80000
-
SDS-PAGE Streptococcus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Streptococcus thermophilus D7P1H2
-
-
6.3.2.3 Streptococcus thermophilus D7P1H2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.2 Ni2+ affinity chromatography Streptococcus thermophilus
6.3.2.3 Ni2+ affinity chromatography Streptococcus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 L-glutamate + L-cysteine + ATP
-
Streptococcus thermophilus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
?
6.3.2.3 gamma-L-glutamyl-L-cysteine + glycine + ATP
-
Streptococcus thermophilus ADP + phosphate + glutathione
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 gamma-glutamylcysteine ligase bifunctional enzyme, also glutathione synthetase activity, EC 6.3.2.3 Streptococcus thermophilus
6.3.2.2 StGCL-GS
-
Streptococcus thermophilus
6.3.2.3 Glutathione synthetase bifunctional enzyme, also gamma-glutamylcysteine ligase activity, EC 6.3.2.2 Streptococcus thermophilus
6.3.2.3 StGCL-GS
-
Streptococcus thermophilus