BRENDA - Enzyme Database show

Characterisation of lipoxygenase isoforms from olive callus cultures

Williams, M.; Harwood, J.L.; Phytochemistry 69, 2532-2538 (2008)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.043
-
linoleic acid
55fold purified enzyme, at pH 8.8, isoform 2
Olea europaea
1.13.11.12
0.08
-
linoleic acid
48fold purified enzyme, at pH 8.8, isoform 1
Olea europaea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
95000
-
Western blotting and gel filtration
Olea europaea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Olea europaea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.12
by ammonium sulphate precipitation and ion-exchange chromatography, 55fold with a yield of 5.6%
Olea europaea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
callus
-
Olea europaea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.11.12
10.2
-
crude extract, with linoleic acid as substrate
Olea europaea
1.13.11.12
12
-
crude extract, with alpha-linolenic acid as substrate
Olea europaea
1.13.11.12
474
-
55fold purified enzyme, with alpha-linolenic acid as substrate
Olea europaea
1.13.11.12
564
-
55fold purified enzyme, with linoleic acid as substrate
Olea europaea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenic acid + O2
-
706121
Olea europaea
?
-
-
-
?
1.13.11.12
linoleic acid + O2
-
706121
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
8
-
for alpha-linolenic acid
Olea europaea
1.13.11.12
8.8
-
for linoleic acid
Olea europaea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.12
7.8
8.4
at pH 7.8, ca. 50% of maximal activity, at pH 8.4, ca. 30% of maximal activity, substrate alpha-linoleic acid
Olea europaea
1.13.11.12
8.5
8.9
at pH 8.5, ca. 30% of maximal activity, at pH 8.9, ca. 80% of maximal activity, substrate linolenic acid
Olea europaea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.12
0.043
-
linoleic acid
55fold purified enzyme, at pH 8.8, isoform 2
Olea europaea
1.13.11.12
0.08
-
linoleic acid
48fold purified enzyme, at pH 8.8, isoform 1
Olea europaea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
95000
-
Western blotting and gel filtration
Olea europaea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
by ammonium sulphate precipitation and ion-exchange chromatography, 55fold with a yield of 5.6%
Olea europaea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.12
callus
-
Olea europaea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.13.11.12
10.2
-
crude extract, with linoleic acid as substrate
Olea europaea
1.13.11.12
12
-
crude extract, with alpha-linolenic acid as substrate
Olea europaea
1.13.11.12
474
-
55fold purified enzyme, with alpha-linolenic acid as substrate
Olea europaea
1.13.11.12
564
-
55fold purified enzyme, with linoleic acid as substrate
Olea europaea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenic acid + O2
-
706121
Olea europaea
?
-
-
-
?
1.13.11.12
linoleic acid + O2
-
706121
Olea europaea
(9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
8
-
for alpha-linolenic acid
Olea europaea
1.13.11.12
8.8
-
for linoleic acid
Olea europaea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.12
7.8
8.4
at pH 7.8, ca. 50% of maximal activity, at pH 8.4, ca. 30% of maximal activity, substrate alpha-linoleic acid
Olea europaea
1.13.11.12
8.5
8.9
at pH 8.5, ca. 30% of maximal activity, at pH 8.9, ca. 80% of maximal activity, substrate linolenic acid
Olea europaea