BRENDA - Enzyme Database show

Oxylipin formation in Nostoc punctiforme (PCC73102)

Lang, I.; Feussner, I.; Phytochemistry 68, 1120-1127 (2007)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.12
expression in Escherichia coli
Nostoc punctiforme
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
70891
-
x * 70891, calculated from sequence
Nostoc punctiforme
1.13.11.33
70891
-
x * 70891, calculated from sequence
Nostoc punctiforme
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Nostoc punctiforme
B2IZG6
PCC73102
-
1.13.11.33
Nostoc punctiforme
B2IZG6
PCC73102
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
alpha-linolenate is the preferred substrate
706116
Nostoc punctiforme
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
gamma-linolenate + O2
-
706116
Nostoc punctiforme
(6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
-
706116
Nostoc punctiforme
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate is higher than 80:20 at all pH values tested
-
-
?
1.13.11.12
additional information
the enzyme also converts arachidonate to its (15S)-hydroperoxide. The enzyme shows no substrate preference
706116
Nostoc punctiforme
?
-
-
-
-
1.13.11.33
arachidonate + O2
-
706116
Nostoc punctiforme
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
-
-
?
1.13.11.33
additional information
the enzyme also converts linoleate to (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate, alpha-linolenate to (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate and gamma-linolenate to (6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate
706116
Nostoc punctiforme
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.13.11.12
?
x * 70891, calculated from sequence
Nostoc punctiforme
1.13.11.33
?
x * 70891, calculated from sequence
Nostoc punctiforme
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
4.5
8.5
broad optimum with a maximum at pH 8.0, substrate: linoleate
Nostoc punctiforme
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.12
expression in Escherichia coli
Nostoc punctiforme
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.12
70891
-
x * 70891, calculated from sequence
Nostoc punctiforme
1.13.11.33
70891
-
x * 70891, calculated from sequence
Nostoc punctiforme
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
alpha-linolenate is the preferred substrate
706116
Nostoc punctiforme
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate
-
-
-
?
1.13.11.12
gamma-linolenate + O2
-
706116
Nostoc punctiforme
(6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate
-
-
-
?
1.13.11.12
linoleate + O2
-
706116
Nostoc punctiforme
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
the ratio of (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate to (9Z,11E,13R)-13-hydroperoxy-9,11-octadecadienoate is higher than 80:20 at all pH values tested
-
-
?
1.13.11.12
additional information
the enzyme also converts arachidonate to its (15S)-hydroperoxide. The enzyme shows no substrate preference
706116
Nostoc punctiforme
?
-
-
-
-
1.13.11.33
arachidonate + O2
-
706116
Nostoc punctiforme
(5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate
-
-
-
?
1.13.11.33
additional information
the enzyme also converts linoleate to (9Z,11E,13S)-13-hydroperoxy-11,13-octadecadienoate, alpha-linolenate to (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate and gamma-linolenate to (6Z,9Z,11E,13S)-13-hydroperoxy-6,9,11-octadecatrienoate
706116
Nostoc punctiforme
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.13.11.12
?
x * 70891, calculated from sequence
Nostoc punctiforme
1.13.11.33
?
x * 70891, calculated from sequence
Nostoc punctiforme
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.12
4.5
8.5
broad optimum with a maximum at pH 8.0, substrate: linoleate
Nostoc punctiforme