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Literature summary extracted from
- Senescence-induced loss in photosynthesis enhances cell wall beta-glucosidase activity (2010), Physiol. Plant., 138, 346-355.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | cell wall | - |
Arabidopsis thaliana | 5618 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 58000 | - |
SDS-PAGE | Arabidopsis thaliana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | ammonium sulfate precipitation, Concanavalin A-Sepharose column chromatography, Shodex-phenyl HIC-814 column chromatography, and Sephacryl S-200 gel filtration | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 0.9 | - |
crude extract, at pH 5.5 and 50°C | Arabidopsis thaliana |
| 3.2.1.21 | 148.5 | - |
after 164.5fold purification, at pH 5.5 and 50°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 2-nitrophenyl beta-D-glucopyranoside + H2O | 83% activity compared to 4-nitrophenyl-beta-D-glucopyranoside | Arabidopsis thaliana | 2-nitrophenol + D-glucopyranose | - |
? |  |
| 3.2.1.21 | 4-nitrophenyl beta-D-glucopyranoside + H2O | 100% activity | Arabidopsis thaliana | 4-nitrophenol + D-glucopyranose | - |
? | |
| 3.2.1.21 | additional information | the enzyme does not hydrolyze 4-nitrophenyl-beta-D-galactoside, cellobiose, D-salicin, and galacturonic acid | Arabidopsis thaliana | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
- |
Arabidopsis thaliana |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 30 | 60 | - |
Arabidopsis thaliana |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 60 | 90 | about 50% activity at 60°C, about 30% activity at 70°C, about 10% activity at 80°C, no activity at 90°C | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.5 | - |
- |
Arabidopsis thaliana |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | 6.5 | - |
Arabidopsis thaliana |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.21 | Arabidopsis thaliana | senescence-induced loss in photosynthesis enhances cell wall beta-glucosidase activity | up |
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Release 2023.1 (January 2023)
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