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Literature summary extracted from

  • Klein, M.; Papenbrock, J.
    Kinetics and substrate specificities of desulfo-glucosinolate sulfotransferases in Arabidopsis thaliana (2009), Physiol. Plant., 135, 140-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.2.38 overexpression of isozyme SOT16 from ecotype C24 in Escherichia coli Arabidopsis thaliana
2.8.2.38 overexpression of isozymes SOT17 from ecotype C24 in Escherichia coli Arabidopsis thaliana
2.8.2.38 overexpression of the isozymes SOT18 from ecotype C24 and ecotype Columbia-0 in Escherichia coli Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.2.38 additional information
-
 additional information kinetics, overview Arabidopsis thaliana
2.8.2.38 0.043
-
 desulfo-4-methylthiobutylglucosinolate pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0 Arabidopsis thaliana
2.8.2.38 0.055
-
 desulfo-3-methylthiopropylglucosinolate pH 9.0, 37°C, isozyme SOT18, ecotype Columbia-0 Arabidopsis thaliana
2.8.2.38 0.065
-
 desulfo-4-methylthiobutylglucosinolate pH 9.0, 37°C, isozyme SOT17 Arabidopsis thaliana
2.8.2.38 0.07
-
 desulfo-3-methylthiopropylglucosinolate pH 9.0, 37°C, isozyme SOT16 Arabidopsis thaliana
2.8.2.38 0.08
-
 desulfo-4-methylthiobutylglucosinolate pH 9.0, 37°C, isozyme SOT16 Arabidopsis thaliana
2.8.2.38 0.088
-
 desulfo-3-methylthiopropylglucosinolate pH 9.0, 37°C, isozyme SOT17 Arabidopsis thaliana
2.8.2.38 0.1
-
 desulfo-3-methylthiopropylglucosinolate pH 9.0, 37°C, isozyme SOT18, ecotype C24 Arabidopsis thaliana
2.8.2.38 0.13
-
 desulfo-4-methylthiobutylglucosinolate pH 9.0, 37°C, isozyme SOT18, ecotype C24 Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.2.38 additional information Arabidopsis thaliana qualitative glucosinolate analysis in leaves of ecotype C24, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.2.38 Arabidopsis thaliana Q9C9C9 isozyme SOT18; ecotypes C24 and Columbia-0, isozyme SOT18
-
2.8.2.38 Arabidopsis thaliana Q9C9D0 isozyme SOT16; ecotype C24, isozyme SOT16
-
2.8.2.38 Arabidopsis thaliana Q9FZ80 isozyme SOT17; ecotype C24, isozyme SOT17
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.2.38 recombinant isozyme SOT16 from Escherichia coli by affinity chromatography Arabidopsis thaliana
2.8.2.38 recombinant isozyme SOT17 from Escherichia coli by affinity chromatography Arabidopsis thaliana
2.8.2.38 recombinant isozymes SOT18 from Escherichia coli by affinity chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.2.38 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-3-methylthiopropyl glucosinolate
-
 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 3-methylthiopropyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-4-methylthiobutyl glucosinolate
-
 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 4-methylthiobutyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate
-
 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-7-methylthioheptyl glucosinolate preferred substrate of isozyme SOT18 of ecotype C24 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 7-methylthioheptyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate
-
 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-8-methylthiooctyl glucosinolate preferred substrate of isozyme SOT18 of ecotype C24 Arabidopsis thaliana adenosine-3',5'-bisphosphate + 8-methylthiooctyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate
-
 Arabidopsis thaliana adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
-
 ?
2.8.2.38 3'-phosphoadenylyl sulfate + desulfo-indol-3-yl-methyl glucosinolate preferred substrate of isozyme SOT16 of ecotype C24 Arabidopsis thaliana adenosine-3',5'-bisphosphate + indol-3-yl-methyl glucosinolate
-
 ?
2.8.2.38 additional information qualitative glucosinolate analysis in leaves of ecotype C24, overview Arabidopsis thaliana ?
-
 ?
2.8.2.38 additional information ecotype C24 isozyme SOT18 prefers the long-chain desulfoglucosinolates, 7-methylthioheptyl and 8-methylthiooctyl, derived from methionine. The substrate specificty of the isozyme SOT18 from ecotype Columbia-0 differs from the SOT18 from ecotype C24, detailed overview. Substrate synthesis and optimization of the enzyme assay method, overview Arabidopsis thaliana ?
-
 ?
2.8.2.38 additional information ecotype C24 isozyme substrate specificities: isozyme SOT17 shows an increased specific activity with increasing chain length of desulfoglucosinolates derived from methionine. Substrate synthesis and optimization of the enzyme assay method, overview Arabidopsis thaliana ?
-
 ?
2.8.2.38 additional information ecotype C24 isozyme substrate specificities: preference of isozyme SOT16 for the indolic desulfoglucosinolate indol-3-yl-methylglucosinolate. Substrate synthesis and optimization of the enzyme assay method, overview Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.2.38 desulfo-Gl SOT
-
 Arabidopsis thaliana
2.8.2.38 desulfo-glucosinolate sulfotransferase
-
 Arabidopsis thaliana
2.8.2.38 ds-Gl SOT
-
 Arabidopsis thaliana
2.8.2.38 SOT
-
 Arabidopsis thaliana
2.8.2.38 SOT16
-
 Arabidopsis thaliana
2.8.2.38 SOT17
-
 Arabidopsis thaliana
2.8.2.38 SOT18
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.2.38 37
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.2.38 9
-
-
 Arabidopsis thaliana