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Literature summary extracted from

  • Duran-Reyes, G.; Pascoe-Lira, D.; Garcia-Macedo, R.; Medina-Navarro, R.; Rosales-Torres, A.M.; Vergara-Onofre, M.; Foyo-Niembro, E.; Gutierrez-Rodriguez, M.E.; Garcia-Gutierrez, M.T.; Valladares-Salgado, A.; Kumate, J.; Cruz, M.
    O-GlcNAc-selective-N-acetyl-beta-D-glucosaminidase activity and mRNA expression in muscle is related to glucosamine-induced insulin resistance (2010), Pharmacology, 85, 121-130.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.169 additional information GlcN is found to provoke hyperglycemia. The insulin resistance index (HOMA-IR) increased. The beta-cell function index (HOMA-beta) diminished. Insulin release index (insulin/glucose) is less in the GlcN group than in the control. Muscle O-GlcNAcase activity diminished Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.169 GalNAc an inhibitor of other hexosaminidases Rattus norvegicus
3.2.1.169 additional information GlcN-induced whole-body insulin resistance is related to tissue O-GlcNAcase activity and mRNA expression. O-GlcNAcase activity is posttranslationally inhibited during GlcN-induced insulin resistance Rattus norvegicus
3.2.1.169 O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino-N-phenyl carbamate PUGNAc Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.169 0.998
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide in the pancreas of rats treated with GlcN Rattus norvegicus
3.2.1.169 1
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the control, pancreas of rats treated with saline Rattus norvegicus
3.2.1.169 1.12
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the control, muscles of rats treated with saline Rattus norvegicus
3.2.1.169 1.24
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide in the liver of rats treated with GlcN Rattus norvegicus
3.2.1.169 1.29
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide compared to the control, liver of rats treated with saline Rattus norvegicus
3.2.1.169 1.51
-
4-nitrophenyl N-acetyl-beta-D-glucosaminide in the muscels of rats treated with GlcN Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.169 additional information Rattus norvegicus removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.169 Rattus norvegicus
-
Sprague-Dawley male rats. GlcN (30 micromol/kg/min) or physiological saline (control) is intravenously infused into the rats for 2 h
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.169 liver
-
Rattus norvegicus
-
3.2.1.169 muscle
-
Rattus norvegicus
-
3.2.1.169 pancreas
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.169 additional information
-
in the rats treated with GlcN, muscle O-GlcNAcase activity diminished (0.28 vs. 0.36 nmol of p-nitrophenyl/mg protein/min) Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.169 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
-
Rattus norvegicus 4-nitrophenol + N-acetyl-beta-D-glucosamine
-
?
3.2.1.169 additional information removes O-N-acetyl-glucosamine residues from O-GlcNAcylated proteins Rattus norvegicus ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.169 O-GlcNAc-selective-N-acetyl-beta-D-glucosaminidase
-
Rattus norvegicus
3.2.1.169 O-GlcNAcase
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.169 37
-
assay at Rattus norvegicus