Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miranda, M.R.; Canepa, G.E.; Bouvier, L.A.; Pereira, C.A.
    Trypanosoma cruzi nucleoside diphosphate kinase 1 (TcNDPK1) has a broad nuclease activity (2008), Parasitology, 135, 1661-1666.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.6 expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Trypanosoma cruzi
-
 ADP + NTP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Trypanosoma cruzi Q4E256
-
-
2.7.4.6 Trypanosoma cruzi Q4E4R9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6 Ni2+-agarose resin column chromatography, gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + NDP
-
 Trypanosoma cruzi ADP + NTP
-
 ?
2.7.4.6 additional information NDPK1 exhibits nuclease activity cleaving not only linear DNA, but also supercoiled plasmid DNA, ATP or ADP do not affect the nuclease activity while the absence of Mg2+ completely inhibits this activity Trypanosoma cruzi ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.6 NDPK1
-
 Trypanosoma cruzi
2.7.4.6 NDPK2
-
 Trypanosoma cruzi
2.7.4.6 nucleoside diphosphate kinase 1
-
 Trypanosoma cruzi
2.7.4.6 nucleoside diphosphate kinase 2
-
 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.6 ATP
-
 Trypanosoma cruzi