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Literature summary extracted from

  • Battsetseg, B.; Boldbaatar, D.; Battur, B.; Xuan, X.; Fujisaki, K.
    Cloning and molecular characterization of tick kynurenine aminotransferase (HlKAT) from Haemaphysalis longicornis (Acari: Ixodidae) (2009), Parasitol. Res., 105, 669-679.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.7 expressed as a glutathione S-transferase fusion protein in soluble form Escherichia coli DH5alpha cells Haemaphysalis longicornis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.7 0.000217
-
 2-oxobutyrate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 0.00438
-
 2-oxo-4-methylthiobutyrate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 0.0175
-
 pyruvate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 0.1116
-
 L-kynurenine in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 0.2062
-
 3-hydroxykynurenine in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.7 43100
-
 gel filtration Haemaphysalis longicornis
2.6.1.7 45000
-
 SDS-PAGE Haemaphysalis longicornis
2.6.1.7 46480
-
 calculated from amino acid sequence Haemaphysalis longicornis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.7 additional information Haemaphysalis longicornis the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.7 Haemaphysalis longicornis B1B6U5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.7 glutathione-Sepharose column chromatography Haemaphysalis longicornis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.7 embryo
-
 Haemaphysalis longicornis
-
2.6.1.7 midgut
-
 Haemaphysalis longicornis
-
2.6.1.7 ovary
-
 Haemaphysalis longicornis
-
2.6.1.7 salivary gland
-
 Haemaphysalis longicornis
-
2.6.1.7 synganglion
-
 Haemaphysalis longicornis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.7 3-hydroxykynurenine + pyruvate
-
 Haemaphysalis longicornis ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxo-4-methylthiobutyrate
-
 Haemaphysalis longicornis ?
-
 ?
2.6.1.7 L-kynurenine + 2-oxobutyrate
-
 Haemaphysalis longicornis ?
-
 ?
2.6.1.7 L-kynurenine + pyruvate
-
 Haemaphysalis longicornis kynurenic acid + L-alanine + H2O
-
 ?
2.6.1.7 additional information the purified recombinant KAT shows dose-dependent inhibition effect on the growth of equine babesial parasite, Babesia caballi, in in vitro culture Haemaphysalis longicornis ?
-
 ?
2.6.1.7 additional information no activity towards L-tryptophan and pyruvic acid Haemaphysalis longicornis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.7 KAT
-
 Haemaphysalis longicornis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.7 30
-
-
 Haemaphysalis longicornis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.7 16 55
-
 Haemaphysalis longicornis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.7 55
-
 the enzymatic activities starts to decrease dramatically when the temperature is above 55°C Haemaphysalis longicornis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.7 0.0018
-
 2-oxobutyrate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 0.16
-
 2-oxo-4-methylthiobutyrate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 2.02
-
 pyruvate in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 55.5
-
 L-kynurenine in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 65.12
-
 3-hydroxykynurenine in 200 mM phosphate buffer, pH 7.5, at 37°C Haemaphysalis longicornis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.7 6 8
-
 Haemaphysalis longicornis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.7 4 9
-
 Haemaphysalis longicornis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.7 pyridoxal 5'-phosphate a highly conserved pyridoxal 5'-phosphate binding site (Gly-Ser-Ala-Gly-Lys-Thr-Phe-Ser) is located between Gly-219 and Ser-226 Haemaphysalis longicornis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.7 Haemaphysalis longicornis calculated from amino acid sequence
-
 7.4

Expression

EC Number Organism Comment Expression
2.6.1.7 Haemaphysalis longicornis protein expression levels increase during the blood-feeding course up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.7 8.2
-
 2-oxobutyrate in 200 mM phosphate buffer, pH7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 36.8
-
 alpha-keto-gamma-methylthiobutyrate in 200 mM phosphate buffer, pH7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 116
-
 pyruvate in 200 mM phosphate buffer, pH7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 315
-
 3-hydroxykynurenine in 200 mM phosphate buffer, pH7.5, at 37°C Haemaphysalis longicornis
2.6.1.7 500
-
 L-kynurenine in 200 mM phosphate buffer, pH7.5, at 37°C Haemaphysalis longicornis