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Literature summary extracted from

  • Imada, M.; Kawashima, S.; Kanehisa, M.; Takeuchi, T.; Asai, T.
    Characterization of alpha-phosphoglucomutase isozymes from Toxoplasma gondii (2010), Parasitol. Int., 59, 206-210.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.2.2 alpha-D-Glucose 1,6-bisphosphate the maximum activity is obtained in the presence of more than 0.2 mM alpha-D-glucose 1,6-bisphosphate Toxoplasma gondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.2 expressed in Escherichia coli BL21(DE3) cells Toxoplasma gondii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.2 0.19
-
 alpha-D-glucose 1-phosphate isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii
5.4.2.2 3.53
-
 alpha-D-glucose 1-phosphate isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.2 Mg2+ Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-I Toxoplasma gondii
5.4.2.2 Mg2+ Mg2+ are the most effective divalent cations for the enzyme activity of isozyme PGM-II Toxoplasma gondii

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.2 Toxoplasma gondii C6L2E2 isozyme PGM-I
-
5.4.2.2 Toxoplasma gondii C6L2F4 isozyme PGM-II
-
5.4.2.2 Toxoplasma gondii Beverley C6L2E2 isozyme PGM-I
-
5.4.2.2 Toxoplasma gondii Beverley C6L2F4 isozyme PGM-II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.2 glutathione Sepharose column chromatography Toxoplasma gondii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.2.2 tachyzoite
-
 Toxoplasma gondii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.2 84
-
 isozyme PGM-II, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii
5.4.2.2 338
-
 isozyme PGM-I, using alpha-D-glucose 1-phosphate as substrate, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Toxoplasma gondii alpha-D-glucose 6-phosphate
-
 ?
5.4.2.2 alpha-D-glucose 1-phosphate
-
 Toxoplasma gondii Beverley alpha-D-glucose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.2.2 alpha-phosphoglucomutase
-
 Toxoplasma gondii
5.4.2.2 PGM-I isozyme Toxoplasma gondii
5.4.2.2 PGM-II isozyme Toxoplasma gondii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.2 93
-
 alpha-D-glucose 1-phosphate isozyme PGM-II, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii
5.4.2.2 398
-
 alpha-D-glucose 1-phosphate isozyme PGM-I, in 50 mM HEPES-KOH, pH 8.0, at 37°C Toxoplasma gondii