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Literature summary extracted from

  • Wong, J.H.; Sahni, U.; Li, Y.; Chen, X.; Gervay-Hague, J.
    Synthesis of sulfone-based nucleotide isosteres: identification of CMP-sialic acid synthetase inhibitors (2009), Org. Biomol. Chem., 7, 27-29.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.43
-
 Neisseria meningitidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 CDP 1 mM, 37°C, pH 8.5, 15 min, 14% inhibition Neisseria meningitidis
2.7.7.43 saturated sulfo-CDP 1 mM, 37°C, pH 8.5, 15 min, 55% inhibition Neisseria meningitidis
2.7.7.43 saturated sulfo-UDP 1 mM, 37°C, pH 8.5, 15 min, 35% inhibition Neisseria meningitidis
2.7.7.43 saturated sulfo-UDP ester 1 mM, 37°C, pH 8.5, 15 min, 24% inhibition Neisseria meningitidis
2.7.7.43 sulfo-CDP 1 mM, 37°C, pH 8.5, 15 min, 47% inhibition Neisseria meningitidis
2.7.7.43 sulfo-CTP 1 mM, 37°C, pH 8.5, 15 min, 39% inhibition Neisseria meningitidis
2.7.7.43 sulfo-UDP 1 mM, 37°C, pH 8.5, 15 min, 6% inhibition Neisseria meningitidis
2.7.7.43 sulfo-UTP 1 mM, 37°C, pH 8.5, 15 min, 33% inhibition Neisseria meningitidis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.43 cytoplasm
-
 Neisseria meningitidis 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + sialic acid Neisseria meningitidis
-
 CMP-sialic acid + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Neisseria meningitidis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Neisseria meningitidis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + sialic acid
-
 Neisseria meningitidis CMP-sialic acid + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMP-sialic acid synthetase
-
 Neisseria meningitidis
2.7.7.43 CSS
-
 Neisseria meningitidis
2.7.7.43 NmCSS
-
 Neisseria meningitidis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 37
-
 inhibition assay Neisseria meningitidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 8.5
-
 inhibition assay Neisseria meningitidis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 14% inhibition Neisseria meningitidis CDP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 24% inhibition Neisseria meningitidis saturated sulfo-UDP ester
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 33% inhibition Neisseria meningitidis sulfo-UTP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 35% inhibition Neisseria meningitidis saturated sulfo-UDP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 39% inhibition Neisseria meningitidis sulfo-CTP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 47% inhibition Neisseria meningitidis sulfo-CDP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 55% inhibition Neisseria meningitidis saturated sulfo-CDP
2.7.7.43 additional information
-
 1 mM, 37°C, pH 8.5, 15 min, 6% inhibition Neisseria meningitidis sulfo-UDP

General Information

EC Number General Information Comment Organism
2.7.7.43 physiological function CMP-sialic acid synthetase is a key enzyme in the biosynthesis of the capsular polysaccharides required for bacterial infection Neisseria meningitidis