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BRENDA support

Literature summary extracted from

  • Sopaci, S.B.; Simsek, I.; Tural, B.; Volkan, M.; Demir, A.S.
    Carboligation reactions with benzaldehyde lyase immobilized on superparamagnetic solid support (2009), Org. Biomol. Chem., 7, 1658-1664.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.38 benzaldehyde no activity with 200 mg m/L benzaldehyde. Reaction conducted with 50 mg benzaldehyde results in a 90% conversion after 45 min synthetic construct

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 expressed in Escherichia coli BL21(DE3)pLysS containing the pUC19-BAL-HIS construct synthetic construct

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.38 Benzoin product inhibition synthetic construct
4.1.2.38 additional information immobilization of BAL to surface-modified magnetic particles with affinity ligand binding synthetic construct

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+
-
 synthetic construct

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 synthetic construct
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38 by Ni2+-NTA affinity column, to purity synthetic construct

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 benzaldehyde + benzaldehyde immobilized BAL mediated reaction, conversion is low synthetic construct (R)-benzoin i.e. 2-hydroxy-1,2-diphenylethan-1-one ?
4.1.2.38 additional information (R)-benzoin and acetaldehyde are converted in an immobilized BAL mediated reaction to 2-hydroxy-1,2-diphenylethan-1-one and (R)-2-hydroxy-1-phenylpropanone synthetic construct ?
-
 ?
4.1.2.38 p-anisaldehyde + dimethoxy acetaldehyde immobilized BAL mediated reaction synthetic construct (R)-2-hydroxy-3,3-dimethoxy-1-(4-methoxyphenyl)propan-1-one
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 synthetic construct
4.1.2.38 benzaldehyde lyase
-
 synthetic construct

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate
-
 synthetic construct