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Literature summary extracted from

  • Pohlmann, V.; Marahiel, M.A.
    delta-Amino group hydroxylation of L-ornithine during coelichelin biosynthesis (2008), Org. Biomol. Chem., 6, 1843-1848.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.195 gene cchB, overexpression of N-terminally thioredoxin-tagged enzyme in Escherichia coli strain BL21(DE3) Streptomyces coelicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.195 additional information
-
additional information Michaelis-Menten kinetics Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.195 L-ornithine + NADPH + H+ + O2 Streptomyces coelicolor
-
N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
1.14.13.195 L-ornithine + NADPH + H+ + O2 Streptomyces coelicolor A3(2)
-
N5-hydroxy-L-ornithine + NADP+ + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.195 Streptomyces coelicolor
-
gene cchB
-
1.14.13.195 Streptomyces coelicolor A3(2)
-
gene cchB
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.13.195 flavoprotein
-
Streptomyces coelicolor

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.195 recombinant N-terminally thioredoxin-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Streptomyces coelicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.195 L-ornithine + NADPH + H+ + O2
-
Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
1.14.13.195 L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
1.14.13.195 L-ornithine + NADPH + H+ + O2
-
Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
1.14.13.195 L-ornithine + NADPH + H+ + O2 absolutely specific for L-ornithine Streptomyces coelicolor A3(2) N5-hydroxy-L-ornithine + NADP+ + H2O
-
?
1.14.13.195 additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor ?
-
?
1.14.13.195 additional information no activity with D-ornithine, N5-formylornithine, L-lysine, L-glutamate, L-glutamine, L-valine, and the tetrapeptide D-Orn-D-Thr-L-Orn-D-Orn, the enzyme performs NADPH oxidation with formation of H2O2, substrate specificity, overview Streptomyces coelicolor A3(2) ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.195 CchB
-
Streptomyces coelicolor

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.195 25
-
assay at Streptomyces coelicolor

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.195 8.5
-
-
Streptomyces coelicolor

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.195 6 9.5 activity range, pH profile Streptomyces coelicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.195 FAD
-
Streptomyces coelicolor
1.14.13.195 NADPH
-
Streptomyces coelicolor

General Information

EC Number General Information Comment Organism
1.14.13.195 physiological function the enzyme is required for synthesis of the nonproteinogenic amino acids N5-hydroxyornithine and N5-hydroxyformylornithine, that are required for iron assembly by the organism Streptomyces coelicolor