Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Tsutani, Y.; Yoshida, K.; Sanada, Y.; Wada, Y.; Konishi, K.; Fukushima, M.; Okada, M.
    Decreased orotate phosphoribosyltransferase activity produces 5-fluorouracil resistance in a human gastric cancer cell line (2008), Oncol. Rep., 20, 1545-1551.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.10 52000
-
 Western blot analyis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.10 MKN-45 cell parent gastric cancer cell line Homo sapiens
-
2.4.2.10 additional information MKN-45/F2R cell, 5-fluorouracil resistant gastric cancer cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 5-fluorouridine 5'-phosphate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 OPRT
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.2.10 Homo sapiens mRNA expression of OPRT is markedly decreased to 12.4% and activity to 46.9% in the 5-fluorouracil resistant MKN-45/F2R cell line compared to the MKN-45 parent cell line. OPRT gene expression is markedly decreased to 14.4% when down-regulated by siRNA down

General Information

EC Number General Information Comment Organism
2.4.2.10 malfunction decreased activity of OPRT plays an important role in the acquired resistance of gastric cancer cells towards 5-fluorouracil. OPRT-knockout MKN-45 parent cells using small interfering RNA demonstrate 15.8fold increased resistance to 5-fluorouracil compared to the control cell Homo sapiens